WormBase Tree Display for Expr_pattern: Expr2407

Expr2407 Expression_of (2)
  Expression_data: Life_stage: WBls:0000024
      WBls:0000003
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
    Anatomy_term: WBbt:0005767 Certain
      WBbt:0005772 Certain
      WBbt:0006873 Certain
    GO_term: GO:0045177
      GO:0016471
  Subcellular_localization: VHA-6 was preferentially localized in dot-like structures in P2 cells of 4-cell stage embryos. VHA-6 was clearly detectable on the apical surface of intestinal cells, forming two lines along the worm body.
  Type: Antibody: Polyconal rabbit antibody. A 350-bp (Glu-69 to Leu-180 of VHA-6) cDNA fragment was inserted into pGEX5X-1(Amersham Pharmacia Biotech). The recombinant proteins expressed in Escherichia coli were used to immunize rabbits, and the antisera were affinity-purified using the same recombinant protein.
  Pattern: After the comma stage in embryos, expression of VHA-6 changed. In 2-fold embryos, VHA-6 was limited to the intestine. Such intestinal expression was also observed in larvae and adults. The C. elegans intestine comprises a tube formed from 20 cells with microvilli on their apical surface. Furthermore, VHA-6 was clearly found at the junction between the pharynx and the intestine.
    VHA-6 was preferentially localized in dot-like structures in P2 cells of 4-cell stage embryos. Only single cells continued to express VHA-6 during further development. After the cleavage stage (~300 cells), when intestinal cells appear, VHA-6 was detected in two distinct cell types. These two cell types were aligned on both lateral sides at the comma stage. The expression pattern of VHA-6 was similar to the cell-specific distribution of P granules, which are passed onto a germ line cell lineage from P2 cells.
  Picture: WBPicture0000008168
  Reference: WBPaper00004842
  Antibody_info: WBAntibody00000406