WormBase Tree Display for Expr_pattern: Expr2407
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| Expr2407 | Expression_of | Gene | WBGene00006915 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006915 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0005767 | Certain | ||
| WBbt:0005772 | Certain | |||
| WBbt:0006873 | Certain | |||
| GO_term | GO:0045177 | |||
| GO:0016471 | ||||
| Subcellular_localization | VHA-6 was preferentially localized in dot-like structures in P2 cells of 4-cell stage embryos. VHA-6 was clearly detectable on the apical surface of intestinal cells, forming two lines along the worm body. | |||
| Type | Antibody | Polyconal rabbit antibody. A 350-bp (Glu-69 to Leu-180 of VHA-6) cDNA fragment was inserted into pGEX5X-1(Amersham Pharmacia Biotech). The recombinant proteins expressed in Escherichia coli were used to immunize rabbits, and the antisera were affinity-purified using the same recombinant protein. | ||
| Pattern | After the comma stage in embryos, expression of VHA-6 changed. In 2-fold embryos, VHA-6 was limited to the intestine. Such intestinal expression was also observed in larvae and adults. The C. elegans intestine comprises a tube formed from 20 cells with microvilli on their apical surface. Furthermore, VHA-6 was clearly found at the junction between the pharynx and the intestine. | |||
| VHA-6 was preferentially localized in dot-like structures in P2 cells of 4-cell stage embryos. Only single cells continued to express VHA-6 during further development. After the cleavage stage (~300 cells), when intestinal cells appear, VHA-6 was detected in two distinct cell types. These two cell types were aligned on both lateral sides at the comma stage. The expression pattern of VHA-6 was similar to the cell-specific distribution of P granules, which are passed onto a germ line cell lineage from P2 cells. | ||||
| Picture | WBPicture0000008168 | |||
| Reference | WBPaper00004842 | |||
| Antibody_info | WBAntibody00000406 |
