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Resources » Reagent

  • Schema

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  • My Library

  • Comments on reagents (0)

  • Clone Information

    There are seven different types of "Clone" objects in WormBase. Although WormBase does not provide clones many of these can be ordered directly from independent sources.

    Type Nomenclature
    Cosmid A*, B*, C*, D*, F*,J*,K*,M*,R*,T*,W*,Z*
    Fosmid H* WRM*
    YAC Y*
    cDNA yk*, EC*, EB*, OST*, CK*, EF*, CEE*, CEM*, CES*, CB*, CN*, cm*
    Plasmid: PCR clones V*,EGAP*
    Other telo clones, 1 BAC, plasmid

    C. elegans Genomic Cosmid:


    No Longer available

    Fosmids:

    C. elegans fosmids from the Moerman library

    No longer available from Source BioScience LifeSciences. Availability of Don Moerman's C.elegans fosmid library is unknown.

    Nomenclature

    Example WRM prefix Plate No. Filter (a,b,c,d) Plate Position
    WRM069bF08 WRM 069 b F08
    WRM069cF08 WRM 069 c F08
    WRM069dF08 WRM 069 d F08

    Library info: (Don Moerman) Vancouver Fosmid Library
    Library Details C. elegans Fosmid Library
    Library Plate numbers 061-069 0610-0641 (41)
    Number of clones 12874 (verified) 15744 (total)
    Plate Format 384
    Source material nematode total organism
    Strain N2
    Vector pCC1FOS(Epicentre)
    Host maintained at low copy number until induced.
    Average insert size 43.3kB
    Cloning site  ?
    Genomic coverage 5.74X (86.7% correct paired ends)
    Creator Don Moerman et al
    Stock center N/A



    C. elegans fosmid from the Incyte Genomics Inc. fosmid library.

    This is an old fosmid library that was lost for quite some time. 29 of these fosmides were used in the C. elegans sequencing project to fill holes left in the Cosmid assembly.

    These clones can be requested from: No longer available

    Nomenclature

    Example H prefix Plate No. Well pos.
    H27D07 H 27 d07
    H30E23 H 30 e23



    Library info: Easy to Screen High-Density Filters, created 1 November 2000, sold by Incyte Genomics Inc.
    Library Details Fosmid C. elegans
    Library Plate numbers 1-43, 1-5
    Number of clones 16,512
    Plate Format 384
    Source material nematode total organism
    Strain N2
    Vector pFOS1
    Host XL1 Blu MR
    Average insert size 40 kb
    Cloning site BamHI
    Genomic coverage 6.6x
    Creator Stephanie Chissoe
    Stock center G.S.C. St. Louis // The Sanger Institute


    YAC

    Please submit all requests for C.elegans genomic clones via Sanger Clone Requests

    Details are then automatically forwarded to [mailto:team178g@sanger.ac.uk Sample Management]. Please do not send request directly to this address.

    Please remember to include a FedEx/DHL Account number to which we can charge the shipping cost. Note that the suffixes to YAC names (e.g. Y73B3A, Y73B3B) indicate sequenced segments of the YAC (Y73B3 in this case). They do not exist as subclones. You will be sent the whole YAC. --Alan Coulson, 2003

    cDNA

    .....

    Plasmid

    • AddGene maintains and supplies a large number of worm plasmid sequences
    • Browse their current list of worm plasmids

    .....

    Other

    .....


    Last edited by Paul Davis – 3 years ago

  • Overview

    This section of the website has been copied over from the WormBase wiki, please use the wiki version of this page for additional details.

    WormBase Wiki Reagents


    Last edited by Paul Davis – 3 years ago

  • Balancers

    Access a table of balancers adapted from the WormBook chapter Genetic Balancers by Edgley et al.


    Last edited by Mary Ann Tuli – 10 years ago

  • Microarrays


    Last edited by Abigail Cabunoc – 13 years ago

  • Strains

    About The Caenorhabditis Genetics Center

    The Caenorhabditis Genetics Center (the CGC) collects, maintains, and distributes stocks of C. elegans.

    The CGC is supported by the National Institutes of Health - National Center for Research Resources. The CGC has been in operation since 1978, first at the University of Missouri, Columbia and since 1992 at the University of Minnesota, Twin Cities.

    Ann Rougvie is the director; Aric Daul is the curator.

    Searching For Strains

    WormBase maintains a list of strains available from the CGC, as well as those noted in the literature on our species' strain pages.
    See the C. elegans strain page here.
    Browse or download the full list of strains from the CGC directly.

    Ordering Strains

    Strains can be ordered directly from the CGC.
    guide to ordering strains for instructions.
    Access the order form here.

    Submitting Strains

    If you have strains you'd wish to share with the community, please submit them to the CGC.

    Acknowledging the CGC

    Please be certain to acknowledge the CGC to help ensure its continued existence.

    Wild Caenorhabditis strains and Non-Caenorhabditis species

    The CGC also maintains stocks of wild Caenorhabditis strains as well as some non-Caenorhabditis species.

    Gene Knockout Strains

    The following projects generate and provide strains that contain knock outs of individual genes.
    * Million Mutation Project
    * National Bioresource Project (Japan)
    * NemaGENETAG Mos insertions


    Last edited by Mary Ann Tuli – 9 years ago