WormBase Tree Display for Variation: WBVar02154209

WBVar02154209 Evidence: Curator_confirmed: WBPerson1983
  Name: Public_name: ve651
    HGVSg: CHROMOSOME_X:g.9331123_9336351del
  Sequence_details: SMap: S_parent: Sequence: T20B5
    Flanking_sequences: aaggggagggaaaacagttgaggacttttg gaatgcgcatacttgatggaaaacccgctc
    Mapping_target: T20B5
    Type_of_mutation: Deletion
    SeqStatus: Sequenced
  Variation_type: Engineered_allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00049390
    Production_method: CRISPR_Cas9
    Status: Live
  Affects: Gene: WBGene00020595
    Transcript: T20B5.2.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        cDNA_position: ?-753
        CDS_position: ?-675
        Protein_position: ?-225
        Intron_number: 2-8/9
        Exon_number: 1-9/10
  Remark: Whole gene deletions made by the Rougvie, Moerman, Hutter and Sternberg labs that replace the coding sequence with a [LoxP + myo-2p::GFP::unc-54 3Prime UTR + rps-27p::neoR::unc-54 3Prime UTR + LoxP] cassette.
    Allele Description:T20B5.2(ve651[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP])
    sgRNA1:aacagttgaggacttttggt sgRNA2: ATCAAGTATGCGCATTCGCT
  Method: Engineered_allele