WormBase Tree Display for Variation: WBVar02125005

WBVar02125005 Evidence: Paper_evidence: WBPaper00042479
  Name: Public_name: db99
  Sequence_details: Mapping_target: Y48D7A
    SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00000308
    Laboratory: AX
    Status: Live
  Affects: Gene: WBGene00001461
  Description: Phenotype: WBPhenotype:0000012 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: Table 1 Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0000022 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0000130 Paper_evidence: WBPaper00053388
        Curator_confirmed: WBPerson46589
        Remark: Figure 5C, Increased dauer entry in flp mutants Paper_evidence: WBPaper00053388
            Curator_confirmed: WBPerson46589
        Phenotype_assay: Strain: WBStrain00000308 Paper_evidence: WBPaper00053388
              Curator_confirmed: WBPerson46589
          Control_strain: WBStrain00000001 Paper_evidence: WBPaper00053388
              Curator_confirmed: WBPerson46589
          Treatment: Animals were exposed to crude pheremone to induce entry into the dauer larva state Paper_evidence: WBPaper00053388
              Curator_confirmed: WBPerson46589
      WBPhenotype:0000542 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0001005 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0001184 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: flp-18(db99) mutant animals exhibited 1.5- to 2-fold higher intestinal fat staining compared to N2 wild-type animals when assayed with Nile Red (Figures 1B and 1C). To confirm our results, we grew animals on fluorescent BODIPY-labeled fatty acid analogs, which are incorporated into fat droplets. BODIPY-labeled flp-18 mutants also exhibited a 1.5- to 2-fold increase in intestinal fat (Figure 1D). Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0001716 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002163 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: To investigate this possibility, we compared oxygen consumption of N2 and flp-18 animals. Whereas N2 animals consumed 1.15 0.17 mol O<sub>2</sub> / (min*mg protein) (SEM), flp-18 animals consumed significantly less, 0.77 0.15 mol O<sub>2</sub> / (min*mg protein) (SEM; paired Student's t test p value = 0.03). Increased intestinal fat in flp-18 mutants thus correlates with reduced aerobic metabolism. Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0002284 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002288 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002296 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002299 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002301 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002309 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002312 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002319 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002323 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002337 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002346 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0004022 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0004023 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
    Phenotype_not_observed: WBPhenotype:0000032 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: flp-18(db99) mutants were viable, developed normally, and appeared healthy. Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0000062 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: flp-18(db99) mutants were viable, developed normally, and appeared healthy. Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0000207 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: Increased intestinal fat in flp-18 mutants was not associated with changes in the rates of feeding, defecation, growth, or egg production (Table S2 and Figure S1). Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0000518 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: flp-18(db99) mutants were viable, developed normally, and appeared healthy. Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0000646 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: flp-18 mutants also had wild-type brood sizes and were not sluggish (data not shown; see below). Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0000673 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: flp-18 mutants also had wild-type brood sizes and were not sluggish (data not shown; see below). Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0001006 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: Increased intestinal fat in flp-18 mutants was not associated with changes in the rates of feeding, defecation, growth, or egg production (Table S2 and Figure S1). Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
      WBPhenotype:0001905 Paper_evidence: WBPaper00033064
        Curator_confirmed: WBPerson2987
        Remark: Increased intestinal fat in flp-18 mutants was not associated with changes in the rates of feeding, defecation, growth, or egg production (Table S2 and Figure S1). Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
        Variation_effect: Predicted_null_via_sequence: Paper_evidence: WBPaper00033064
            Curator_confirmed: WBPerson2987
  Reference: WBPaper00043908
    WBPaper00033064
    WBPaper00042479
    WBPaper00053388
    WBPaper00064927
  Method: Allele