WormBase Tree Display for Variation: WBVar00088492
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| WBVar00088492 | Evidence | Paper_evidence | WBPaper00013464 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Name | Public_name | lg601 | |||||||
| Sequence_details | Mapping_target | K04E7 | |||||||
| Type_of_mutation | Deletion | ||||||||
| SeqStatus | Pending_curation | ||||||||
| Variation_type | Allele | ||||||||
| Origin | Species | Caenorhabditis elegans | |||||||
| Strain | WBStrain00003894 | ||||||||
| Laboratory | XY | ||||||||
| Status | Live | ||||||||
| Affects | Gene | WBGene00003877 | |||||||
| Interactor | WBInteraction000523966 | ||||||||
| WBInteraction000523967 | |||||||||
| WBInteraction000536825 | |||||||||
| WBInteraction000536826 | |||||||||
| WBInteraction000536827 | |||||||||
| WBInteraction000536828 | |||||||||
| WBInteraction000536829 | |||||||||
| WBInteraction000536832 | |||||||||
| Isolation | Mutagen | UV/TMP | |||||||
| Description | Phenotype | WBPhenotype:0000119 | Paper_evidence | WBPaper00045460 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "75% of these 20 proteins (Figure 2A; Table S2) revealed decreased levels in pept-1 as compared to levels in wild-type animals, whereas only 5 proteins displayed increased levels." | Paper_evidence | WBPaper00045460 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000120 | Paper_evidence | WBPaper00045460 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "While ATP synthase subunit alpha H28016.1 (Q9XXK1) did not show any significant regulation in the proteomics data set or the western blot analysis, heat shock protein HSP-60 (P50140) was significantly downregulated in pept-1, as was also observed in western blot analysis. Furthermore, the low expression of two proteins involved in C1 metabolism, S-adenosyl methionine synthetase SAMS-1 (O17680) and S-adenosyl homocysteine hydrolase AHCY-1 (P27604), confirmed earlier data from our group... Using the KEGG pathway mapper, 15 out of the 30 proteins were assigned as ribosomal components (Figure S1), all of them showing a reduced protein abundance in pept-1 when compared to that in wild-type. The diminished protein synthesis capacity might explain the observed decline in protein expression between 20 and 40 h of postembryonal development in pept-1 but not in wild-type animals... 75% of these 20 proteins (Figure 2A; Table S2) revealed decreased levels in pept-1 as compared to levels in wild-type animals, whereas only 5 proteins displayed increased levels... Interestingly, prominent changes in unfolded protein response (UPR) terms 'ER nuclear signaling pathway' and 'response to endoplasmic reticulum stress' (p value = 1.34 x 10^-4, Figure 2C) with downregulation of 5 genes of the heat shock-protein family (C12C8.1, T27E4.3/ T27E4.9, Y46H3A.2, T27E4.2, and T22A3.2) were observed... Several genes, of which induction by tunicamycin is dependent on xbp-1 and ire-1, were found to be downregulated in pept-1 (e.g., T14G8.3, Y41C4A.11, K01D12.11, B0285.9, and ZC239.12)." | Paper_evidence | WBPaper00045460 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000137 | Paper_evidence | WBPaper00045460 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Furthermore, the loss of pept-1 strongly reduced expression of rsks-1 (p70S6 kinase), whereas daf-15 mRNA (raptor) expression remained unchanged (Figure 4C,D)." | Paper_evidence | WBPaper00045460 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000694 | Paper_evidence | WBPaper00055920 | |||||||
| Curator_confirmed | WBPerson2603 | ||||||||
| WBPhenotype:0000964 | Paper_evidence | WBPaper00027611 | |||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Affected_by | Molecule | WBMol:00000896 | Paper_evidence | WBPaper00027611 | |||||
| Curator_confirmed | WBPerson712 | ||||||||
| WBPhenotype:0001834 | Paper_evidence | WBPaper00045460 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "While the total amount of xbp-1 mRNA did not change in response to loss of pept-1, the abundance of the spliced transcript decreased significantly (Figure 3C,D)." | Paper_evidence | WBPaper00045460 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | GO_term | GO:0030968 | PATO:0000460 | Paper_evidence | WBPaper00045460 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0002255 | Paper_evidence | WBPaper00045460 | |||||||
| Curator_confirmed | WBPerson2603 | ||||||||
| Remark | amino acid deficiency; "Analysis of cytosolic amino acid concentrations revealed that the content of almost all free amino acids was decreased in pept-1 compared to that in wild-type (Figure 4A). Only cysteine levels showed no significant changes, and glutamine and serine concentrations were significantly elevated." | Paper_evidence | WBPaper00045460 | ||||||
| Curator_confirmed | WBPerson2603 | ||||||||
| Phenotype_not_observed | WBPhenotype:0000114 | Paper_evidence | WBPaper00045460 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Furthermore, the loss of pept-1 strongly reduced expression of rsks-1 (p70S6 kinase), whereas daf-15 mRNA (raptor) expression remained unchanged (Figure 4C,D)." | Paper_evidence | WBPaper00045460 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Disease_info | Models_disease | DOID:9970 | |||||||
| Models_disease_in_annotation | WBDOannot00000178 | ||||||||
| Reference | WBPaper00027611 | ||||||||
| WBPaper00013464 | |||||||||
| WBPaper00045460 | |||||||||
| WBPaper00055920 | |||||||||
| Remark | 1.7kb deletion, which lacks 257bp of the promoter, the translational start codon, and the first 6 exons of the pep-2 gene | Paper_evidence | WBPaper00013464 | ||||||
| Method | Deletion_allele |
