WormBase Tree Display for Variation: WBVar00088295

WBVar00088295 Name: Public_name: ku1
  Sequence_details: SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00026496
    Laboratory: MH
    Status: Live
  Affects: Gene: WBGene00003401
    Interactor: WBInteraction000518949
      WBInteraction000524422
  Genetics: Mapping_data: In_multi_point: 2223
        2224
        2225
        2226
  Description: Phenotype: WBPhenotype:0000099 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: impenetrant defects in P6.p lineage Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
        Penetrance: Incomplete: Person_evidence: WBPerson261
              Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0008125 PATO:0000460 Person_evidence: WBPerson261
                Curator_confirmed: WBPerson712
      WBPhenotype:0000386 Paper_evidence: WBPaper00033433
        Curator_confirmed: WBPerson2987
        Remark: Copper-induced germ cell apoptosis was abolished in mpk-1(ku1) mutants (Figure 4). Paper_evidence: WBPaper00033433
            Curator_confirmed: WBPerson2987
        Affected_by: Molecule: WBMol:00002862 Paper_evidence: WBPaper00033433
              Curator_confirmed: WBPerson2987
        Phenotype_assay: Treatment: Worms were exposed to 10 micromolar of copper for 12 hours Paper_evidence: WBPaper00033433
              Curator_confirmed: WBPerson2987
      WBPhenotype:0000411 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: rod-like early larval lethality phenotypes Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
      WBPhenotype:0000640 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: cs Egl Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Cold_sensitive: Person_evidence: WBPerson261
              Curator_confirmed: WBPerson712
      WBPhenotype:0000651 Paper_evidence: WBPaper00024311
        Curator_confirmed: WBPerson712
        Remark: Animals become severely constipated compared to wild-type worms when grown on lawns of M. nematophilum, whereas they are not constipated when grown on standard E. coli food source, OP50. Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        Penetrance: Incomplete: Paper_evidence: WBPaper00024311
              Curator_confirmed: WBPerson712
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were grown on NGM plates seeded with trace (usually 0.01% (v/v)) M. nematophilum in OP50. Strains were cultured on these plates at 25*C. Paper_evidence: WBPaper00024311
              Curator_confirmed: WBPerson712
      WBPhenotype:0000698 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: variable Vul Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
      WBPhenotype:0001013 Paper_evidence: WBPaper00024311
        Curator_confirmed: WBPerson712
        Remark: The severety of constipation induced in animals grown in the presence of M. nematophilum suggests these animals are hypersensitive to infection by this pathogen. Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        Penetrance: Incomplete: Paper_evidence: WBPaper00024311
              Curator_confirmed: WBPerson712
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were grown on NGM plates seeded with trace (usually 0.01% (v/v)) M. nematophilum in OP50. Strains were cultured on these plates at 25*C. Paper_evidence: WBPaper00024311
              Curator_confirmed: WBPerson712
      WBPhenotype:0001413 Paper_evidence: WBPaper00024311
        Curator_confirmed: WBPerson712
        Remark: Animals failed to swell when exposed to Microbacterium nematophilum, although bacteria were present in the rectum of these animals. Bacteria were visualized by nucleic acid dye SYTO 13. Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were grown on NGM plates seeded with trace (usually 0.01% (v/v)) M. nematophilum in OP50. Strains were cultured on these plates at 25*C. Paper_evidence: WBPaper00024311
              Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000347 Paper_evidence: WBPaper00024311
        Curator_confirmed: WBPerson712
        Remark: Expression is unaltered for two hindgut cell markers, egl-5 and mab-9. Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0005800 PATO:0000460 Paper_evidence: WBPaper00024311
                Curator_confirmed: WBPerson712
      WBPhenotype:0000386 Paper_evidence: WBPaper00035318
        Curator_confirmed: WBPerson2987
        Remark: Cobalt-induced germline cell apoptosis was unaffected in mpk-1(ku1) mutants (Figure 3) Paper_evidence: WBPaper00035318
            Curator_confirmed: WBPerson2987
        Affected_by: Molecule: WBMol:00003058 Paper_evidence: WBPaper00035318
              Curator_confirmed: WBPerson2987
        Phenotype_assay: Treatment: Synchronized young adult hermaphrodites were treated in K-medium with 0.01 millimolar Cobalt chloride for 12 hours, and apoptotic cells were scored after AO staining. Paper_evidence: WBPaper00035318
              Curator_confirmed: WBPerson2987
      WBPhenotype:0000717 Paper_evidence: WBPaper00024311
        Curator_confirmed: WBPerson712
        Remark: Expression is unaltered for two hindgut cell markers, egl-5 and mab-9. Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00024311
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0005800 PATO:0000460 Paper_evidence: WBPaper00024311
                Curator_confirmed: WBPerson712
      WBPhenotype:0001173 Paper_evidence: WBPaper00040855
        Curator_confirmed: WBPerson557
        Remark: Adult males show appropriate cell death of the linker cell. Paper_evidence: WBPaper00040855
            Curator_confirmed: WBPerson557
      WBPhenotype:0001278 Paper_evidence: WBPaper00057074
        Curator_confirmed: WBPerson712
        Remark: neurons did not show a loss in unc-25::GFP expression Paper_evidence: WBPaper00057074
            Curator_confirmed: WBPerson712
        Image: WBPicture0000014913 Paper_evidence: WBPaper00057074
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0005027 PATO:0000460 Paper_evidence: WBPaper00057074
                Curator_confirmed: WBPerson712
            WBbt:0005021 PATO:0000460 Paper_evidence: WBPaper00057074
                Curator_confirmed: WBPerson712
      WBPhenotype:0001660 Paper_evidence: WBPaper00006052
        Curator_confirmed: WBPerson2021
        Remark: No disruption of ASE asymmetry (as seen with lim-6 reporters) Paper_evidence: WBPaper00006052
            Curator_confirmed: WBPerson2021
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00006052
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: otIs114, otIs6 Paper_evidence: WBPaper00006052
              Curator_confirmed: WBPerson2021
      WBPhenotype:0002378 Paper_evidence: WBPaper00054670
        Curator_confirmed: WBPerson2706
        Remark: animals avoid OP50 lawns contaminated with Microbacterium nematophilum as wild type Paper_evidence: WBPaper00054670
            Curator_confirmed: WBPerson2706
  Reference: WBPaper00040855
    WBPaper00006052
    WBPaper00023380
    WBPaper00033433
    WBPaper00024311
    WBPaper00035318
    WBPaper00054670
    WBPaper00057074
  Method: Allele