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WormBase Tree Display for Transgene: WBTransgene00021260

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Name Class

WBTransgene00021260Public_nameqnEx95
Summary[Phsp-16.2:nlp-22; Pmyo-2:mCherry, unc-119(+)]
ConstructionConstructWBCnstr00020887
Coinjection_otherPmyo-2::mCherry, unc-119(+)]
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0000019Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Remark (10)
Caused_by_geneWBGene00003760Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Phenotype_assayGenotypekin-2(ce179)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
unc-31(e928)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
egl-3(nu349)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
egl-21(n476)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
egl-4(n479)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
ceh-17(np1)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
nmur-1(ok1387)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
nmur-2(ok3502)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
nmur-3(ok2295)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
nmur-4(ok1381)Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
WBPhenotype:0001524Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
RemarkWhen authors overexpressed nlp-22 using an inducible heatshock promoter (Fig. 2a) during the normally active adult period, they observed quiescence of feeding and locomotion (Fig. 2b, c, Supplementary Movie 1, Supplementary Table S1). Two hours after heat shock, control animals made 15.9 +/- 7.3 (all values presented as mean +/- s.d.) (N=15) anterior body bends per min, whereas animals overexpressing nlp-22 made 0.55 +/- 1.1 (N=25) body bends per min (Fig. 2b). Wild-type animals and transgenic control animals expressing GFP under the control of a heat-shock promoter pumped 2 h after heat shock at rates of 264.9 +/- 17.4 (N=20) and 273.6 +/- 17.5 (N=20) pumps per min, respectively (Fig. 2c, Supplementary Table S1). Animals carrying the integrated or extrachromosomal Phsp16.2:nlp-22 arrays, qnIs142 or qnEx95, pumped at rates of 18.6 +/- 31.1 (N=37) and 25.2 +/- 32.3 (N=40) per min, respectively, 2 h after heat shock (Fig. 2c, Supplementary Table S1).Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00003760Paper_evidenceWBPaper00044546
Curator_confirmedWBPerson2987
ReferenceWBPaper00044546
SpeciesCaenorhabditis elegans