WormBase Tree Display for Transgene: WBTransgene00020486
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WBTransgene00020486 | Public_name | ksEx11 | ||||||
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Summary | [Phsp-16::MEK-1(Ser221Glu)(Ser225Glu); dpy-20(+)] | |||||||
Synonym | [pPD49.83-ceMEK-SSEE, pMH86] | |||||||
Construction | Construct | WBCnstr00020122 | ||||||
Coinjection | WBCnstr00019441 | |||||||
Laboratory | FK | |||||||
Construction_summary | pPD49.83-ceMEK-SSEE (50 ng/l) was coinjected with 50 ng/l pMH86 dpy-20(+) marker into dpy-20 animals to obtain a transgenic strain, FK137 dpy-20;ksEx11 [pPD49.83-ceMEK-SSEE, pMH86]. | |||||||
Genetic_information | Extrachromosomal | |||||||
Phenotype | WBPhenotype:0000006 | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | A 30 minute heat shock of strain FK137 at 35 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) led to cessation of egg laying | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003185 | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Treatment | 30 minutes heat shock at 35 degrees Celsius | Paper_evidence | WBPaper00004381 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000747 | Paper_evidence | WBPaper00004381 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Approximately 5 hours after a 60 minute heat shock at 33 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) pharyngeal muscle contraction was suppressed (also see Figure 7) | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003185 | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001213 | Paper_evidence | WBPaper00004381 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | A 30 minute heat shock of strain FK137 at 35 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) led to cessation of spontaneous movement | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003185 | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0002221 | Paper_evidence | WBPaper00004381 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Approximately 5 hours after a 60 minute heat shock at 33 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) vacuoles appeared in the pharynx (Figure 6A) | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003185 | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Treatment | 60 minute heat shock at 33 degrees Celsius | Paper_evidence | WBPaper00004381 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0002222 | Paper_evidence | WBPaper00004381 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Approximately 5 hours after a 60 minute heat shock at 33 degrees Celsius (to induce expression of an activated form of mek-1 from the ksEx11 transgene) vacuoles appeared in the uterus lumen (Figure 6B) | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00003185 | Paper_evidence | WBPaper00004381 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Associated_with | Strain | WBStrain00043016 | ||||||
Reference | WBPaper00004381 | |||||||
Species | Caenorhabditis elegans |