WormBase Tree Display for Transgene: WBTransgene00019358
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| WBTransgene00019358 | Public_name | neSi22 | |
|---|---|---|---|
| Summary | [oma-1::gfp (RNAa), cb-unc-119(+)] | ||
| Construction | Construct | WBCnstr00018695 | |
| Coinjection_other | Cbr-unc-119(+) | ||
| Integration_method | Single_copy_insertion | ||
| Construction_summary | A SpeI-AleI fragment containing oma-1::gfp was excised from pRL475 (gift from R. Lin) and inserted into plasmid B1496 (Shirayama et al., 2012), a modified version of the LGII MosSCI targeting vector pCFJ151 (Frokjaer-Jensen et al., 2008) to obtain targeting plasmid M1001. A DNA mixture containing 50 ng/ul of targeting plasmid M1001 was injected into WM186, and a modified MosSCI heat shock method (Shirayama et al., 2012) was used to generate the single-copy insertion line WM288. | ||
| Genetic_information | Integrated | ||
| Map | II | ||
| Associated_with | Strain | WBStrain00046596 | |
| Reference | WBPaper00044619 | ||
| WBPaper00053722 | |||
| WBPaper00061478 | |||
| Species | Caenorhabditis elegans |
