WormBase Tree Display for Transgene: WBTransgene00015019
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WBTransgene00015019 | Public_name | wuEx187 | ||||||
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Summary | [Pftn-1::ftn-1::ftn-1 3'UTR; coel::GFP] | |||||||
Construction | Construct | WBCnstr00014621 | ||||||
Coinjection_other | coel::GFP | |||||||
Genetic_information | Extrachromosomal | |||||||
Phenotype | WBPhenotype:0001236 | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | The presence of the wuEx187 [Pftn-1::ftn-1::ftn-1 3'UTR + coel::GFP] transgene resulted in increased expression of the Pftn-1::GFP transgene (data not shown) | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00001500 | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | Pftn-1::GFP | Paper_evidence | WBPaper00040914 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001663 | Paper_evidence | WBPaper00040914 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Animals overexpressing ftn-1 from the wuEx187 transgene [Pftn-1::ftn-1::ftn-1 3'UTR; coel::GFP] and exposed to 10 millimolar tert-butyl hydroperoxide (t-BOOH) exhibited increased life span compared to wild type worms also exposed to t-BOOH (Figure 2I, Table S7) | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00001500 | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Affected_by | Molecule | WBMol:00003239 | Paper_evidence | WBPaper00040914 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Treatment | Animals were exposed to 10 millimolar tert-butyl hydroperoxide (t-BOOH) at room temperature and assayed for survival | Paper_evidence | WBPaper00040914 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0002249 | Paper_evidence | WBPaper00040914 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | ftn-1 overexpression from the wuEx187 transgene [Pftn-1::ftn-1::ftn-1 3'UTR; coel::GFP] reduced protein oxidation levels by 31% (p = 0.03) (Figures 2G, 2H). | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00001500 | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_not_observed | WBPhenotype:0000039 | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | ftn-1 overexpression from the wuEx187 transgene [Pftn-1::ftn-1::ftn-1 3'UTR; coel::GFP] did not affect life span (Figure 2J). | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00001500 | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Temperature | 20 | Paper_evidence | WBPaper00040914 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000673 | Paper_evidence | WBPaper00040914 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Overexpression of ftn-1 from the wuEx187 [Pftn-1::ftn-1::ftn-1 3'UTR + coel::GFP] transgene did not significantly affect brood size (Figure 2F) | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00001500 | Paper_evidence | WBPaper00040914 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Used_for | Interactor | WBInteraction000517922 | ||||||
Associated_with | Strain | WBStrain00043104 | ||||||
Reference | WBPaper00040914 | |||||||
Species | Caenorhabditis elegans |