WormBase Tree Display for Transgene: WBTransgene00014844
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WBTransgene00014844 | Public_name | xqIs132 | |
---|---|---|---|
Summary | [unc-47::TDP-43-WT; unc-119(+)] | ||
Construction | Construct | WBCnstr00014450 | |
Integration_method | UV | ||
Construction_summary | Human cDNAs were amplified by PCR and cloned into the Gateway vector pDONR221 following the manufacturer's protocol (Invitrogen). Multisite Gateway recombination was performed with the pDONR TDP-43 clone along with clones containing the unc-47 promoter the unc-54 3'UTR plasmid pCM5.37 (Dr. Geraldine Seydoux, Johns Hopkins, Addgene plasmid 17253) and the destination vector pCFJ150 to create the expression vectors. | ||
Genetic_information | Integrated | ||
Reference | WBPaper00040834 | ||
WBPaper00041295 | |||
WBPaper00041343 | |||
WBPaper00045700 | |||
WBPaper00046914 | |||
WBPaper00048916 | |||
WBPaper00053435 | |||
Species | Caenorhabditis elegans |