WormBase Tree Display for Transgene: WBTransgene00008589
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WBTransgene00008589 | Public_name | rtIs26 | |||||
---|---|---|---|---|---|---|---|
Summary | [hsp::osm-11; elt-2p::gfp; pha-1(+)] | ||||||
Synonym | osm-11(OE) | ||||||
Construction | Construct | WBCnstr00008293 | |||||
Coinjection_other | elt-2p::gfp (pJM#67); pha-1(+) (pBX#1) | ||||||
Construction_summary | Clone = pHA#294, pHA#295, and pHA#296. rtIs26 was generated by irradiation of extrachromosomal array rtEx472, which was generated by co-injection of pHA#294, pHA#295, and pHA#296 at 20 ng/l with pJM#67 (elt-2p::gfp) and pBX#1 (pha-1(+)). In the three aforementioned pHA plasmids, the PCR amplified osm-11 cDNA was inserted into NheI/SacI of pPD49.78, allowing heat shock inducible expression of the osm-11 cDNA (using the hsp-16-41 promoter). | ||||||
Genetic_information | Integrated | ||||||
Map | III | ||||||
Phenotype | WBPhenotype:0000641 | Paper_evidence | WBPaper00038400 | ||||
Curator_confirmed | WBPerson712 | ||||||
Remark | After heat-shock, animals displayed bouts of spontaneous and transient quiescence, during which locomotion and pharyngeal pumping ceased for several minutes regardless of the presence of food. | Paper_evidence | WBPaper00038400 | ||||
Curator_confirmed | WBPerson712 | ||||||
Animals exhibited a decreased level of basal locomotion activity, which was significantly enhanced after heat-shock treatment. | Paper_evidence | WBPaper00038400 | |||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_gene | WBGene00003891 | Paper_evidence | WBPaper00038400 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0001072 | Paper_evidence | WBPaper00038400 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | After heat-shock, animals displayed bouts of spontaneous and transient quiescence, during which locomotion and pharyngeal pumping ceased for several minutes regardless of the presence of food. | Paper_evidence | WBPaper00038400 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_gene | WBGene00003891 | Paper_evidence | WBPaper00038400 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0001524 | Paper_evidence | WBPaper00038400 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | With or without heat-shock, animals displayed bouts of spontaneous and transient quiescence, during which locomotion and pharyngeal pumping ceased for several minutes regardless of the presence of food. During this period, animals failed to respond to body touch at a significantly higher percentage of the time than control animals. | Paper_evidence | WBPaper00038400 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_gene | WBGene00003891 | Paper_evidence | WBPaper00038400 | ||||
Curator_confirmed | WBPerson712 | ||||||
Used_for | Interactor | WBInteraction000500650 | |||||
WBInteraction000500651 | |||||||
WBInteraction000500652 | |||||||
WBInteraction000500653 | |||||||
WBInteraction000500654 | |||||||
WBInteraction000500657 | |||||||
Reference | WBPaper00038400 | ||||||
WBPaper00051547 | |||||||
WBPaper00053808 | |||||||
WBPaper00054814 | |||||||
WBPaper00055514 | |||||||
Species | Caenorhabditis elegans |