WormBase Tree Display for RNAi: WBRNAi00101621

WBRNAi00101621 Homol: Homol_homol: T23D8:RNAi
  Sequence_info: PCR_product: sjj_T23D8.9
  Experiment: Laboratory: CT
    Date: 01 Nov 2010 00:00:00
    Genotype: wIs78 [SCMp::GFP marker transgene for seam cells]; lin-42(n1089)
    Treatment: RNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector.
    Temperature: 20
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: T23D8.9a Inferred_automatically: RNAi_primary
      T23D8.9c Inferred_automatically: RNAi_primary
      T23D8.9b Inferred_automatically: RNAi_primary
    Gene: WBGene00006379 Inferred_automatically: RNAi_primary
    Transcript: T23D8.9b.1 Inferred_automatically: RNAi_primary
      T23D8.9c.1 Inferred_automatically: RNAi_primary
      T23D8.9a.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00037838
  Phenotype_not_observed: WBPhenotype:0000168 Remark: RNAi did not significantly affect the precocious production of alae in L4 larvae animals carrying the lin-42(n1089) mutation
  Remark: (Figure 7C) sys-1 RNAi
  Method: RNAi