WormBase Tree Display for RNAi: WBRNAi00101461
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WBRNAi00101461 | Homol | Homol_homol | T23D8:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_T23D8.9 | |||
Experiment | Laboratory | CT | |||
Date | 01 Nov 2010 00:00:00 | ||||
Genotype | wIs78 [SCMp::GFP marker transgene for seam cells] | ||||
Treatment | RNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector. All strains feeding on RNAi bacteria were cultured at 20 degrees Celsius unless otherwise noted. | ||||
Temperature | 20 | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | T23D8.9a | Inferred_automatically | RNAi_primary | |
T23D8.9c | Inferred_automatically | RNAi_primary | |||
T23D8.9b | Inferred_automatically | RNAi_primary | |||
Gene | WBGene00006379 | Inferred_automatically | RNAi_primary | ||
Transcript | T23D8.9b.1 | Inferred_automatically | RNAi_primary | ||
T23D8.9c.1 | Inferred_automatically | RNAi_primary | |||
T23D8.9a.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00037838 | ||||
Phenotype_not_observed | WBPhenotype:0000354 | Remark | sys-1(RNAi) did not have any discernable effect on V seam cell numbers divisions or terminal seam cell number and terminal differentiation. | ||
WBPhenotype:0000746 | Remark | sys-1(RNAi) did not have any discernable effect on V seam cell numbers divisions or terminal seam cell number and terminal differentiation. | |||
WBPhenotype:0001887 | Remark | sys-1(RNAi) did not have any discernable effect on V seam cell numbers divisions or terminal seam cell number and terminal differentiation. | |||
WBPhenotype:0001923 | Remark | sys-1(RNAi) did not have any discernable effect on V seam cell numbers divisions or terminal seam cell number and terminal differentiation. | |||
Remark | (Figure 3A) sys-1 RNAi | ||||
Method | RNAi |