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| WBPicture0000012529 | Description | Figure 5. The achaete-scute homolog hlh-14 is required to specify neurons in the ASE lineage. A: hlh-14 is required for the terminal specification of several neuron pairs from the ABalppp/praaa lineage branch. Images of hlh-14(tm295) mutants indicating a loss of ASE (ASEL: lim- 6prom::gfp, ASER: gcy-5prom::gfp and ASEL/ASER: che-1prom::rfp) OLL (ser-2prom2:gfp) and AFD (gcy-8::gfp) cell fates. Heterozygous balanced siblings are unaffected (presence of balancer indicated by GFP in the pharynx). B: Lineage, 3D-morphogenesis and hlh-14 expression analysis in the ABalppp/praaa lineage of wild-type (containing the ntIs1 transgene), hlh-14(gm34); otIs114, hlh-14(tm295); ntIs1 and hlh-14(tm295); otEx4507, Ex[hlh-14FOS::yfp]; ntIs1 embryos. The left panel shows the division pattern and expression (purple lines) of hlh-14 in the ABalppp/praaa lineage as determined by 4Dlineage analysis. hlh-14 expression was assessed in both gmIs20 and hlh-14(tm295); Ex[hlh-14FOS::yfp] animals. In wild-type embryos the cells divided as expected. In hlh-14(gm34) mutants the 3 most posterior great-granddaughters of the ABalppp/praaa blastomere fail to divide. In hlh-14(tm295) mutants these defects extend to the 4 most posterior granddaughter. The number of lineages that divided as indicated by the lineage and the total number of individual left or right lineages followed to terminal division (and assessed for 3D cell positions) are shown at the bottom of each lineage diagram. The asterisks indicate that on one occasion the lineages still divided but aberrantly, much later than normal. N indicates neurons and G indicates glia. The right panel shows representative examples of the 3D-positions of cells from the ABalppp/ABpraaa lineage at the AB256-cell stage as assessed by 4D-lineage analysis. Both ventral and frontal views are presented. The posterior descendants of the ABalppp/praaa blastomere are dorsally and posteriorly mispositioned in hlh-14 mutants (asterisks), a position normally occupied by hypodermal cells. The fosmid array rescues the division and morphogenesis defects. The gut lineage is indicated for positional information. Cell coloring, except C-lineage, after [10]. The purple triangle under the panel indicates that hlh-14 expression is lost more rapidly in the anterior of the lineage and this correlates with more penetrant defects in posterior parts of lineage in hlh-14 mutants. C: Images of hlh-14 expression in embryos at the pre-morphogenesis (AB256-cell) stage. Top panel shows the expression of hlh-14 from an integrated translational hlh-14::gfp reporter [28]. Bottom panel shows the expression of hlh-14 from a rescuing YFP-tagged hlh-14 fosmid array. Expression in the ASE, PVQ/HSN/PHB, and DVC/PVR lineages is indicated. The full expression pattern of hlh- 14 in the ABalppp/praaa lineage is indicated by purple lines in the lineage diagram in panel C and full expression in the C-lineage is indicated in Figure 6. | |||
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| Name | Fig5.jpg | ||||
| Depict | Expr_pattern | Expr9584 | |||
| Anatomy | WBbt:0005909 | ||||
| WBbt:0006048 | |||||
| WBbt:0006123 | |||||
| WBbt:0006228 | |||||
| WBbt:0006697 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2011 | ||||
| Article_URL | DOI | id | 10.1371/journal.pgen.1002109 | ||
| Journal_URL | PLoSGenetics | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00039776 |
