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WBPicture0000011961 Description: Figure 4. The flp-18 Receptors npr-4 and npr-5 Act in Multiple Tissues to Regulate Fat (A-D) Expression of npr-4p::RFP (red) and DiO (3,30 -dioctadecyloxacarbocyanine, perchlorate)- filled neurons (green). Shown are confocal projec- tions: lateral view of head neurons (A) and ventral view of tail neurons (B; arrowheads indicating PHA and PHB and arrow indicating PQR). Also shown are npr-4 expression in coelomocytes (indi- cated by arrow) and in the intestine (C; confocal image, lateral view) and npr-4 expression in the intestine and the BDU neuron, indicated by an arrow (D; confocal image, lateral view).(E-H) Expression of npr-5p::RFP (red) and DiO filled neurons (green). Shown are confocal projec- tions: lateral view of head neurons (E) and ventral view of tail neurons (F; arrowheads indicating PHA and PHB). Also shown are npr-5 expression in head and neck muscles (G; confocal image, lateral view) and npr-5 expression in body wall muscles. (H; confocal image, lateral view). Scale bars are 10 mm.(I) Quantification of Nile Red fluorescence of L4 animals. Expression of npr-5 selectively, in a group of ciliated neurons, rescues the fat phenotype of npr-5. Ns: N2 = 157, flp-18(db99) = 235, npr- 5(ok1583) = 141, npr-5(ok1583) Ex[tax-4p::npr-5] = 121, npr-5(ok1583) Ex[odr-4p::npr-5] = 53.(J) Quantification of Nile Red fluorescence of L4 animals. Ns: N2 = 75, npr-4 = 157, npr-4(tm1782) Ex[elt-2p::npr-4] = 129. Error bars indicate SEM.
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  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2009
    Article_URL: DOI id 10.1016/j.cmet.2009.02.003
    Journal_URL: CellMetabolism
    Publisher_URL: Elsevier
  Reference: WBPaper00033064