WormBase Tree Display for Picture: WBPicture0000010095

WBPicture0000010095 Description: Figure 3. PRG-1 Protein Is Expressed in the Germline and Required for 21U-RNA Accumulation. (A) Northern blot analysis of 21U-RNA-1, 21U-RNA-3442, and miR-66 expression in wild-type and the indicated homozygous strains. The double mutant was prg-1(tm872); prg-2(tm1094). The SL1 precursor served as a loading control.(B) The PRG-1 developmental expression profile. Protein lysates generated from wild-type populations at distinct developmental stages were analyzed using a western blot (top left), as were protein lysates from wild-type worms and from the mutant strains examined in Figure 2B (top right). Tubulin served as a loading control. Expression of prg-1/prg-2 mRNA was analyzed by quantitative real-time PCR, using actin (act-3) mRNA as the normalization standard (bottom panel).(C-F) PRG-1 immunofluorescence (red) and DNA DAPI staining (blue) in dissected gonad arms from an adult hermaphrodite (C) and male (D), a two-cell embryo (E), and a four-cell embryo (F). In (C) and (D) the mitotic (MPZ) and meiotic zones (transition zone plus pachytene) are indicated, as are the proximal zones containing oocytes and sperm (respectively).(G) Dual immunofluorescence analysis of three oocytes in the proximal arm of a wild-type hermaphrodite gonad stained for PRG-1 and PGL-1 as indicated. Yellow represents overlap in the merged image (bottom panel).
  Name: F3.jpg
  Crop: Crop_picture: WBPicture0000010096
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2008
    Article_URL: DOI id 10.1016/j.molcel.2008.06.002
    Journal_URL: MolecularCell
    Publisher_URL: Elsevier
  Reference: WBPaper00031961