WormBase Tree Display for Expr_pattern: Expr9980
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| Expr9980 | Expression_of | Gene | WBGene00003915 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003915 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0004575 | Certain | |||
| WBbt:0004576 | Certain | |||
| WBbt:0005784 | Certain | |||
| WBbt:0005792 | Certain | |||
| WBbt:0008378 | Certain | |||
| GO_term | GO:0005737 | |||
| GO:0005886 | ||||
| GO:0043186 | ||||
| Subcellular_localization | At the L1 stage, PAN-1 is seen in the pharynx and on the membranes of intestinal cells; PAN-1 also localizes to punctate granules that surround the nuclei of Z2 and Z3, the two germline precursor cells. This perinuclear localization to the germline precursor cells is characteristic of P granules and PAN-1 persists at this location as the wild type larvae mature. As wild type larvae mature, PAN-1 remains localized around the developing germ cell nuclei. Immuno-reactivity of older larvae to PAN-1 antibody revealed a strong, uniform cytoplasmic distribution in somatic tissue, as well as a distinct peri-nuclear pattern for PAN-1 around the germ cell nuclei, confirmed as localizing with P granules by confocal images that show the co-localization of PAN-1 with the P-granule proteins GLH-1 and PGL-1 as also seen for PGL-1 in the L3 larva. | |||
| During embryogenesis, PAN-1 protein is uniformly distributed throughout the cytoplasm of the germline and somatic blastomeres, as seen for pan-1 mRNA, with no obvious concentration of PAN-1 in the P granules. PAN-1 was not seen in P granules at any stage of embryogenesis. | ||||
| In the adult worm PAN-1 is most obvious in the germline P granules shown here in a single gonad arm of an adult hermaphrodite. In confocal images, PAN-1 co-localizes with GLH-1 and PGL-1 throughout the germline, beginning with the distal mitotic region through to the proximal region, which contains maturing oocytes. After oocyte maturation, the nuclear membrane breaks down and PAN-1, as is the case for other P-granule proteins, disperses into the cytoplasm. The perinuclear localization of PAN-1 is most obvious in the meiotic, pachytene region of the germline, as also seen with PGL-1. In the adult male testis, PAN-1 is also present in P granules co-localizing with GLH-1 throughout spermatogenesis. However, PAN-1 is not present in mature sperm. Along with its germline location, PAN-1 is also concentrated in the somatic tissue of the pharynx in adult hermaphrodites, males, and larvae, a presence that is less obvious in the L1 stage and is very obvious in the adult, while PAN-1 reacts at decreased levels in the adult gut. | ||||
| PAN-1 protein was detected throughout the cytoplasm of both somatic and germline tissues in adult hermaphrodite worms, while the pre-immune sera were negative. | ||||
| Type | Antibody | |||
| Pattern | PAN-1 is a P granule component that co-localizes with GLH-1 in the germline from the first larval stage throughout germline development; it is present in P granules surrounding the germ cell nuclei throughout oogenesis, until oocytes mature and P granules disperse in the oocyte cytoplasm, and throughout spermatogenesis, except in mature sperm. In addition, PAN-1 is found in the pharynx and intestine, the somatic tissues associated with molting. | |||
| Reference | WBPaper00040830 | |||
| Antibody_info | WBAntibody00002316 |
