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WormBase Tree Display for Expr_pattern: Expr841

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Name Class

Expr841Expression_ofGeneWBGene00003912
Reflects_endogenous_expression_ofWBGene00003912
Expression_dataLife_stageWBls:0000003
Anatomy_termWBbt:0003822Certain
WBbt:0003833Certain
WBbt:0004292Certain
WBbt:0004409Uncertain
RemarkWeak, transient expression.
WBbt:0004410Uncertain
RemarkWeak, transient expression.
WBbt:0004489
WBbt:0004497Certain
WBbt:0004499Certain
WBbt:0004512Certain
WBbt:0004513Certain
WBbt:0005772Partial
RemarkExpressed in int-5 cells.
WBbt:0005870Certain
WBbt:0005883Certain
WBbt:0005886Certain
WBbt:0005897Certain
WBbt:0005899Certain
WBbt:0005921Certain
WBbt:0005956Certain
WBbt:0005960Certain
WBbt:0005962Certain
WBbt:0005964Certain
WBbt:0005980
WBbt:0005983Certain
WBbt:0005991Certain
WBbt:0006004Certain
WBbt:0006019Certain
WBbt:0006024Certain
WBbt:0006043Certain
WBbt:0006045Certain
WBbt:0006054Certain
WBbt:0006059Certain
WBbt:0006066Certain
WBbt:0006076Certain
WBbt:0006098Certain
WBbt:0006100Certain
WBbt:0006106Certain
WBbt:0006117
WBbt:0006123Certain
WBbt:0006138Certain
WBbt:0006151Certain
WBbt:0006168Certain
WBbt:0006185Certain
WBbt:0006199Certain
WBbt:0006210Certain
WBbt:0006212Certain
WBbt:0006225Certain
WBbt:0006229
WBbt:0006231Certain
WBbt:0006233Certain
WBbt:0006256Certain
WBbt:0006258Certain
WBbt:0006263Certain
WBbt:0006267Certain
WBbt:0006268Certain
WBbt:0006271Certain
WBbt:0006281
WBbt:0006289Certain
WBbt:0006307Certain
WBbt:0006319Certain
WBbt:0006326Certain
WBbt:0006343Certain
WBbt:0006357Certain
WBbt:0006358
WBbt:0006374Certain
WBbt:0006390Certain
WBbt:0006395Certain
WBbt:0006399Certain
WBbt:0006416
WBbt:0006430Certain
WBbt:0006444Certain
WBbt:0006502Certain
WBbt:0006512Certain
WBbt:0006516Certain
WBbt:0006521Certain
WBbt:0006525Certain
WBbt:0006563Certain
WBbt:0006574Certain
WBbt:0006594Certain
WBbt:0006604Certain
WBbt:0006628Certain
WBbt:0006645Certain
WBbt:0006663Certain
WBbt:0006664Certain
WBbt:0006668Certain
WBbt:0006700Certain
WBbt:0006718Certain
WBbt:0006722Certain
WBbt:0006777Uncertain
RemarkWeak, transient expression.
WBbt:0006778Uncertain
RemarkWeak, transient expression.
WBbt:0006779Uncertain
RemarkWeak, transient expression.
TypeAntibodyAnti-PAL-1 antibody.
Reporter_gene
PatternPAL-1 produced from zygotic transcripts is seen initially in C and D lineage cells that also expressed maternally derived PAL-1. As gastrulation begins, expression is seen in only Ca and Cp and then in their daughters, of which 2 are hypodermoblasts (Caa and Cpa) and 2 are myoblasts (Cap and Cpp). The GFP reporter is first detected at the late 2C-cell stage and then more strongly in the 4 daughters. At about 100 cells, expression is also detected in the 2 D-lineage myoblasts. Thereafter, PAL-1 continues to be detected in all C and D descendants until the end of gastrulation at about 350 cells. At about 180 cells (midgastrulation), the C hypodermal precursors, which express more strongly than the muscle precursors, form a characteristic double row on each side of the dorsal midline in the posterior. Thereafter, PAL-1 decreases in these cells and is no longer detectable with antibody after 350 500 cells. At about 250 cells, expression is detected in two AB cells that border the posterior left edge of the mesectodermal cell layer that is closing the ventral gastrulation cleft (ABplpappp and ABplppppp) and slightly later in the right homolog of one of them (ABprppppp). The daughters and granddaughters of these cells, generated after the cleft closes, continue to express strongly along the ventral midline until about the time of hatching. Beginning at about 360 cells, as morphogenesis begins, weak transient expression is detected in the posterior ectodermal P cells and occasionally in posterior V cells as both groups move ventrally. During this period the V cells become the lateral seam cells, and the P cells undergo their terminal embryonic divisions as they complete hypodermal enclosure of the embryo. Meanwhile, in the interior, pal-1 expression, detectable both with antibody and with reporter constructs, appears at about 350 cells in 2 Ea descendents near the middle of the gut primordium (the int5 pair) and in 2 anteriorly located MS descendants which migrate to the posterior and become the mesoblast M and the right intestinal muscle (mu intR). During early morphogenesis as the embryo develops through the comma stage and begins to elongate, all the pal-1-expressing cells (approximately 50) are located in the posterior ventral region, except for the 2 midgut cells which lie more dorsally. The descendants of ABpl/rppppp, as well as mu intR, move into the elongating tail and participate in formation of the rectal and associated intestinal muscles, as well as the ventral tail hypodermis. Expression diminishes during elongation and by hatching is detectable only in the 2 gut cells, M, mu intR, and 10 cells descended from ABpl/rppppp.
RemarkThree methods, lacZ, gfp, antibody staining results all mixed together. Lots of unextracted cell objects buried in pattern text.
ReferenceWBPaper00004499
TransgeneWBTransgene00000285
WBTransgene00000286
Antibody_infoWBAntibody00000128