WormBase Tree Display for Expr_pattern: Expr8396

Expr8396 Expression_of (2)
  Expression_data: GO_term: GO:0005737
      GO:0005768
      GO:0005794
      GO:0016323
      GO:0016324
  Subcellular_localization: Co-staining with FM4-64, which in C. elegans has been shown to label the later part of the endosomal system and lysosomes: TAT-1C-GFP partially colocalized with the dye, although GFP fluorescence was also clearly seen in parts of the cell not labeled by FM4-64. Co-staining with LysoTracker, which predominantly labels acidified lysosomes, revealed that most TAT-1C-GFP fluorescence was not coincident with that of LysoTracker. TAT-1C-GFP colocalized with monomeric red fluorescent protein (mRFP)-RAB-5, a marker for early endosomes and with mRFP-RAB-7, a marker for early and late endosomes. However, TAT-1C appears not to be restricted to early endosomes because colocalization was also seen with RME-1, a marker for recycling endosomes, and with GRASP55, which labels Golgi. In contrast, little overlap was seen between TAT-1C and the endoplasmic reticulum marker HDEL.
    The fusion protein, which efficiently rescued the mutant phenotype, localized to the basolateral and apical cell membrane as well as to many internal structures within the cytoplasm. The relative amount of GFP fluorescence associated with the membrane or with cytoplasm varied somewhat during development: in L1 and L2 animals, most GFP fluorescence was cytoplasmic but in older animals, in which the fluorescence was generally weaker, the plasma membrane was also labeled.
  Type: Reporter_gene
  Remark: Picture: Figure S1, Figure 2F,I.
  Reference: WBPaper00032283
  Transgene: WBTransgene00030495