WormBase Tree Display for Expr_pattern: Expr8160
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| Expr8160 | Expression_of (2) | |
|---|---|---|
| Expression_data (3) | ||
| Subcellular_localization | The GFP was localized mainly in the cytoplasm. The fli-1 expression in the spermatheca was unevenly distributed with strong expression in the distal and proximal part of spermatheca. fli-1 was also expressed within neuronal migratory structures such as axons. In the body-wall muscle, fli-1::gfp was localized in a distinct striated pattern, with accumulation of FLI-1::GFP at dense bodies, which is similar to the Z-disc in the muscle cells in other organisms. The expression pattern of fli-1 in body-wall muscle cells is reminiscent of the distribution of the actin filaments. Authors stained F-actin in body-wall muscle cells with rhodamine-labeled phalloidin and found that it colocalized with FLI-1::GFP. | |
| Type | Reporter_gene | |
| Pattern | The reporter was widely expressed and the expression level was dynamic, varying among tissues and developmental stages. The expression of fli-1::gfp was first detected during embryogenesis before the apparent morphogenesis occurred. In the comma-stage embryo, expression of fli-1::gfp was detected in muscle cells. The expression of fli-1::gfp was not observed before the 32-cell-stage embryos (n > 40 embryos examined). At post-embryonic stages, expression of fli-1::gfp was detected in many tissues, including the pharyngeal muscle, rectum muscle, vulva muscle, proctodeum muscle in males, and somatic gonad tissues (including spermatheca and distal tip cell). | |
| Picture | WBPicture0000010969 | |
| Remark | Picture: Figure 9. | |
| Reference | WBPaper00030927 | |
| Transgene | WBTransgene00030350 |
