WormBase Tree Display for Expr_pattern: Expr7858
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| Expr7858 | Expression_of (2) | |
|---|---|---|
| Expression_data (3) | ||
| Subcellular_localization | The protein was detected as a punctate pattern in the cytoplasm indicating a vesicular localization. At mid-embryogenesis, when epithelialization proceeds, CeVPS-27-positive vesicles were preferentially enriched in hypodermal, pharyngeal and intestinal cells, where they localized close to the apical membrane as revealed by the apical junction marker AJM-1. Later during morphogenesis, CeVPS-27-positive vesicles became more uniformly localized. Several arguments support the idea that CeVPS-27 localizes to endosomes (see Remark). | |
| Type (2) | ||
| Pattern | CeVPS-27 was detected ubiquitously, as early as in the 2-cell embryo and persisted throughout the embryonic and larval development and in adult animals. At mid-embryogenesis, when epithelialization proceeds, CeVPS-27-positive vesicles were preferentially enriched in hypodermal, pharyngeal and intestinal cells. The specificity of the signal was confirmed by its complete disappearance after inactivation of Cevps-27 by RNAi. During larval development and in adults, CeVPS-27 was expressed ubiquitously but remained more abundant in the intestine, the hypodermis, the pharynx and in the adult epithelial tissues; namely the uterus, the vulva, the spermatheca and the gonad. CeVPS-27 could also be found in the excretory canal, and the six scavenger cells called coelomocytes. | |
| Remark (4) | ||
| Reference | WBPaper00026641 | |
| Transgene | WBTransgene00030161 | |
| Antibody_info | WBAntibody00001021 |
