WormBase Tree Display for Expr_pattern: Expr3918
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| Expr3918 | Expression_of | Gene | WBGene00004855 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004855 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005733 | Certain | |||
| WBbt:0005772 | Certain | |||
| GO_term | GO:0005737 | |||
| GO:0016324 | ||||
| Subcellular_localization | During early morphogenesis, as the hypodermal cells migrate to enclose the embryo, SMA-1 is present at all epithelial cell boundaries, with some protein in the cytoplasm. Once enclosure is complete, SMA-1 protein localizes to the apical membrane of hypodermal, pharyngeal, and gut cells where it remains during embryonic elongation. SMA-1 is found in a speckled pattern at the apical surface of the both dorsal/ventral and seam hypodermis, with increased intensity near cell boundaries. The reorganization of SMA-1 in the hypodermis is concurrent with a change in the organization of actin, from cortical arrays to parallel bundles associated with the apical membrane. | |||
| Type | Antibody | |||
| Pattern | SMA-1 spectrin first appears in hypodermal cell precursors located on the dorsal surface of the embryo prior to enclosure. SMA-1 is present in the hypodermal cells as they enclose the embryo, and continues to be expressed in the dorsal, lateral, and ventral hypodermal cells throughout embryogenesis. SMA-1 is also expressed in gut and pharynx cells prior to embryonic enclosure, and throughout embryogenesis. In sma-1(ru18) null mutant embryos, both immunoblot and in situ immunofluorescence staining show no signal, demonstrating the antiserum is specific for SMA-1 protein. | |||
| Remark | No detailed description on expression pattern in other life stages. | |||
| The protein localization patterns observed using SMA-1 antisera are consistent with mRNA in situ expression data (see Expr1425), except in the excretory canal cell, where sma-1 mRNA is present during embryogenesis, but SMA-1 protein expression is delayed until the L1 stage. The timing of SMA-1 protein expression precisely correlates with the period when the excretory cell begins to rapidly elongate, during the first larval stage of development. | ||||
| Reference | WBPaper00025184 | |||
| Antibody_info | WBAntibody00001017 | |||
| WBAntibody00001018 |
