WormBase Tree Display for Expr_pattern: Expr3463
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| Expr3463 | Expression_of (2) | |||
|---|---|---|---|---|
| Homol | Homol_homol | T07H8:Expr | ||
| Expression_data | Anatomy_term | WBbt:0003937 | Certain | |
| WBbt:0003939 | Certain | |||
| WBbt:0004070 | Certain | |||
| WBbt:0004098 | Certain | |||
| WBbt:0004102 | Certain | |||
| WBbt:0004991 | Certain | |||
| WBbt:0004993 | Certain | |||
| WBbt:0005237 | Certain | |||
| WBbt:0005796 | Certain | |||
| GO_term | GO:0043005 | |||
| GO:0005783 | ||||
| Subcellular_localization | Expression of a full-length MEC-1::GFP protein fusion from the mec-1 promoter rescued the mec-1(e1496) null phenotype and revealed that MEC-1 was processed differently in touch receptor neurons. The fusion protein was visible in the ER of the same non-touch cells seen with the promoter fusion and in some neurons of the ventral cord, but it was not seen along their processes. In contrast, MEC-1::GFP was consistently visible in the ER and along the processes of the touch receptor neurons. In addition, MEC-1::GFP was sometimes seen along the edge of the body wall muscles. Since these cells did not express the promoter fusion, this muscle fluorescence suggests that MEC-1 is a secreted protein. | |||
| Type | Reporter_gene | |||
| Pattern | The mec-1 promoter expressed GFP in the touch receptor neurons, many lateral neurons (the SDQ, PLN, and ALN neurons and two neurons of the dorsal sublateral cord), the PVT neuron, and the intestinal muscle. | |||
| Picture | WBPicture0000009641 | |||
| WBPicture0000009643 | ||||
| Remark | Because of the expression in non-touch receptor neurons, mec-1 (also tagged with gfp) was tested whether could rescue the Mec phenotype when expressed from the touch receptor neurons-specific mec-18 promoter. Touch receptor expression was sufficient for rescue (three strains produced 90% +/- 5% touch-sensitive animals; N = 70). | |||
| LacZ expression was used to test protein topology with respect to the plasma membrane, because cytoplasmic, but not extracellular, beta-galactosidase is active . When lacZ was fused directly after the DNA for the predicted signal sequence, no beta-galactosidase activity was found, suggesting that the resulting fusion protein is extracellular. Insertion of an artificial transmembrane domain after the signal sequence restored activity. beta-galactosidase activity was also absent when lacZ was fused at codon 45, directly after sequence for the weakly predicted transmembrane domain. As before, insertion of a sequence encoding an artificial transmembrane domain before lacZ restored activity. These results suggest that MEC-1 does not contain a transmembrane domain between residues 21 and 41. | ||||
| Reference | WBPaper00024622 | |||
| Transgene | WBTransgene00028620 |
