WormBase Tree Display for Expr_pattern: Expr3417
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| Expr3417 | Expression_of | Gene (47) | ||
|---|---|---|---|---|
| Reflects_endogenous_expression_of (47) | ||||
| Expression_data | Anatomy_term | WBbt:0005178 | Certain | |
| WBbt:0005319 | Certain | |||
| WBbt:0006760 | Certain | |||
| WBbt:0006794 | Certain | |||
| WBbt:0006797 | Certain | |||
| WBbt:0006798 | Certain | |||
| GO_term | GO:0005938 | |||
| GO:0009986 | ||||
| Subcellular_localization | In mated females, extracellular MSP exhibits a graded distribution, with a sharp boundary between the -1 and -2 oocytes. Fluorescence intensity measurements indicate that MSP is localized in a graded manner from the spermatheca to the oocyte. Fluorescence intensity measurements also indicate that there is significant MSP staining over the -2 and -3 oocytes. | |||
| To pinpoint the localization of MSP at the oocyte cell surface, a 3D confocal analysis of MSP localization was conducted a in mated females using the RME-2 yolk receptor to mark the oocyte plasma membrane and the early endosomal compartments. Three-dimensional image reconstructions of the data indicate that MSP localizes in three regions: (1) in superficial focal planes at the oocyte cell surface with RME-2 just beneath; (2) in the same plane as the RME-2 signal; and (3) within the oocyte beneath the plasma membrane. | ||||
| Using anti-MSP mAbTR-20 and visual inspection, 91% of mated female gonad arms exhibited extracellular MSP localization (n=36), with 39% showing extracellular MSP as far as the most proximal oocyte, and the rest exhibiting extracellular MSP only within the spermatheca. | ||||
| With confocal microscopy, extracellular MSP appeared both punctate and diffuse in the spermatheca, the gonad arm and the uterus. Analysis of 3D data stacks indicated that punctate extracellular MSP was enriched near spermatozoa on the spermathecal walls. The largest puncta were at the diffraction limit of the microscope (0.5 um) and were found nearby spermatozoa. In the proximal gonad arm, MSP was more diffuse and localized in focal plane slices near the oocyte surface. In the uterus, large MSP puncta was observed close to spermatozoa. Diffuse MSP was also observed in extracellular spaces surrounding embryos in the uterus. MSP puncta can be observed near spermatozoa in the uterus and spermatheca using wide-field microscopy, when these regions were less crowded with spermatozoa. | ||||
| Type | Antibody | |||
| Picture | WBPicture0000007641 | |||
| WBPicture0000007642 | ||||
| WBPicture0000007643 | ||||
| Reference | WBPaper00026617 | |||
| Antibody_info | WBAntibody00000038 |
