WormBase Tree Display for Expr_pattern: Expr2942
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| Expr2942 | Expression_of | Gene | WBGene00006944 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006944 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0005733 | |||
| WBbt:0005784 | Certain | |||
| WBbt:0005792 | ||||
| GO_term | GO:0005634 | |||
| GO:0000793 | ||||
| Subcellular_localization | At metaphase, WRN-1 had an unusual location, overlapping with the periphery of equatorially aligned condensed chromosomes facing the spindle poles, and also, less frequently, overlapping with the mitotic spindles. When nuclei in the intestine were magnified, an uneven distribution of the protein was observed. Both mitotic and meiotic prophase germ cells in the L4 stage gonad contained WRN-1 in the nuclei, but the level was significantly reduced in the meiotic prophase germ cells of adults. In the oocytes of adult gonads, WRN-1 localized to condensed chromosomes. | |||
| Type | Antibody | |||
| Pattern | WRN-1 was immunolocalized in C. elegans at various developmental stages. The protein was non-uniformly distributed in nuclei from the early embryonic stage throughout embryogenesis. In early embryos, the protein level was higher in mitotic cells than in interphase cells, as is clearly shown in 6- and 8-cell stage embryos. In larval stages WRN-1 was present in the nuclei of numerous cells, and the fraction of WRN-1-positive somatic cells was significantly lower in adults. In adult worms, the protein was expressed in hypodermal, intestinal and germ cells, and the protein level decreased with age. | |||
| Picture | WBPicture0000007590 | |||
| Remark | RNAi of wrn-1 eliminated immunologically detectable protein in embryos, supporting effective suppression of the endogenous gene expression and no cross-reactivity of the antibody. By contrast, RNAi of a RecQ homolog (RCQ-5) encoded by the E03A35.2 ORF, with 39% amino acid identity in the helicase domain of WRN-1, did not affect WRN-1 expression, thus underlining the specificity of wrn-1 RNAi. | |||
| Reference | WBPaper00024206 | |||
| Antibody_info | WBAntibody00000892 |
