WormBase Tree Display for Expr_pattern: Expr2682
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| Expr2682 | Expression_of | Gene | WBGene00006994 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006994 | ||
| Expression_data | GO_term | GO:0005737 | |
| GO:0005813 | |||
| GO:0000922 | |||
| Subcellular_localization | ZYG-9 exhibits a dynamic cell cycle-dependent distribution pattern. The protein localizes to the meiotic spindle and spindle poles and to the mitotic centrosomes. In metaphase and early anaphase stages, ZYG-9 can be detected over the central mitotic spindle region as well. During interphase, ZYG-9 protein is distributed throughout the cytoplasm. Specificity of this staining pattern was confirmed by its absence in embryos homozygous for any of three strong zyg-9 alleles that lacked the 155-kD protein. | ||
| Type | Antibody | Polyclonal rabbit antibody against recombinant ZYG-9 protein. Fusion protein containing the carboxy-terminal 462 amino acids of the ZYG-9 protein was prepared for polyclonal antibody production. A fragment containing 1,385 base pairs from the 3' end of the zyg-9 cDNA was cloned into the expression vector QE30 (QIAGEN, Inc., Santa Clarita, CA). ZYG-96HIS fusion proteins were expressed in bacteria, inclusion bodies were isolated, and then the expected 51-kD protein was purified by SDS-PAGE followed by electroelution and dialysis. Rabbit polyclonal antibodies were generated at the Cornell Research Animal Resources Facility (Ithaca, NY). ZYG-9specific antibodies were purified from crude serum by column chromatography using ZYG-9 fusion protein coupled to CNBr-activated Sepharose 6MB beads (Pharmacia Biotech., Inc., Piscataway, NJ). | |
| Picture | WBPicture0000008906 | ||
| WBPicture0000008907 | |||
| WBPicture0000008908 | |||
| Reference | WBPaper00003099 | ||
| Antibody_info | WBAntibody00000179 |
