WormBase Tree Display for Expr_pattern: Expr2646
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| Expr2646 | Expression_of | Gene | WBGene00004804 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004804 | ||
| Expression_data (3) | |||
| Subcellular_localization | nuclei | ||
| Type | Reporter_gene | [skn-1::gfp] translational fusion. skn-1 gfp promoter fusion construct (SknPro GFP) was created by ligating GFP vector pPD95.67 and a PCR-amplified 2.1-kb clone containing the promoter region and 38 amino acids from the first ATG codon of the skn-1 gene from cosmid T19E7. To generate the SKN-1 GFP translational fusion construct, the 5.7-kb EcoRI DNA fragment that rescues the maternal skn-1 phenotype and encodes the 533-amino-acid SKN-1 protein was amplified from cosmid B0547. A ClaI site was created immediately 3' to the SKN-1 C terminus by the QuikChange method (Stratagene), which was used for all site-directed mutagenesis. This EcoRI fragment was subcloned into pUC18, which contained the upstream 1.3-kb SphIEcoRI fragment from SknPro GFP. A 0.8-kb ClaI fragment that contained the GFP open reading frame (amplified from plasmid pPD114.35) was then cloned into the ClaI site to generate an in-frame exon fusion of GFP to the SKN-1 C terminus. | |
| Pattern | In larvae and young adults, SKN-1 GFP was usually present at very low levels in intestinal nuclei. SKN-1 GFP was readily detectable in the ASI neurons, but not in other cells in the head. | ||
| In late-stage embryos, SKN-1 GFP was also present in intestinal nuclei but not in the hypodermis. | |||
| Nuclear SKN-1 GFP was uniformly detected in intestinal precursors beginning at the 50100-cell stage, then in both the intestine and hypodermis. | |||
| Picture | WBPicture0000008452 | ||
| Remark | Gene_regulation: A promoter fusion transgene in which only the SKN-1 N terminus was linked to GFP (SknPro GFP) was constitutively expressed at high levels in all intestinal cells, suggesting that SKN-1 expression or localization might also be regulated posttranscriptionally by oxidative stress. | ||
| Gene_regulation: After exposure to either paraquat or heat, neither the location nor intensity of SKN-1 GFP was detectably altered in the ASI neurons, but in a high percentage of animals, elevated levels of SKN-1 GFP appeared in intestinal cell nuclei, particularly anteriorly and posteriorly, where GCS-1 GFP is most robustly expressed. SKN-1 GFP accumulated in intestinal nuclei within 5 min after treatment with 50 mM sodium azide, which induces oxidative stress by blocking mitochondrial electron transport. | |||
| Reference | WBPaper00006024 | ||
| Transgene | WBTransgene00000658 | ||
| WBTransgene00000657 |
