WormBase Tree Display for Expr_pattern: Expr2621
expand all nodes | collapse all nodes | view schema
| Expr2621 | Expression_of (2) | ||
|---|---|---|---|
| Expression_data (3) | |||
| Subcellular_localization | In nid-1(ur41) animals, lam-3 is localized with the mispositioned axons rather than along the normal nerve pathways. This suggests that lam-3 is localized to neuronal cell surfaces by specific lam-3 receptors. | ||
| Type | Antibody | Rabbit and chicken polyclonal antibody. To generate antisera against LAM-3, a plasmid construct was made by subcloning into the vector pQE (Qiagen, CA) an 800 bp cDNA fragment that contains the sequence encoding the G3 domain. The fusion protein produced contain 6HIS-tags and were purified according to the instructions of Qiagen and were used to immunize rabbits. Antisera against each fusion protein were also raised in Chicken. | |
| Pattern (3) | |||
| Picture (2) | |||
| Remark | Colocalization Assay: In order to compare directly the distribution of lam-3 and epi-1, both subunits were co-stained using species-specific secondary antibody conjugates. As the germ layers develop, both subunits are deposited between the layers. However, the staining for laminin A is most intense around the pharyngeal and intestinal precursor cells, whereas staining for laminin B is most intense around the myoblast cells and along epidermal cells. By the onset of elongation, distinct layers of laminin A and laminin B staining can be distinguished, particularly anteriorly between the developing pharynx and the body wall. This indicates that the segregation of the laminin isoforms begins early, before or as organogenesis proceeds. In mnDf90, the two subunits remain differentially localized after elongation. lam-3 is localized to the pharyngeal basement membrane, whereas epi-1 is associated mainly with the body wall basement membranes and only weakly with the pharyngeal basement membrane. These results indicate that each laminin alpha subunit is segregated in the embryo to different adjacent basement membranes and that each membrane retains its unique subunit composition. | ||
| Reference | WBPaper00006014 | ||
| Antibody_info (2) |
