WormBase Tree Display for Expr_pattern: Expr2350
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| Expr2350 | Expression_of | Gene | WBGene00003964 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003964 | ||
| Expression_data (3) | |||
| Subcellular_localization | Gut nuclei did not stain and appeared as dark patches among the PDI-3-labeled gut tissue. DPY-7 antibody was localized solely to the annular furrows of the hypodermally derived cuticle. | ||
| Type | Antibody | Rabbit polyconal peptide antibody against PDI-3. A PDI-3-specific anti-peptide antiserum was produced in rabbits using the synthetic peptide CREVKDFVSFISKHSTDGLKGFS. The cysteine residue was added to permit conjugation to keyhole limpet hemocyanin prior to immunization. | |
| Pattern | In L1 larvae, PDI-3 localized in many tissues with predominant staining found in the hypodermal and the gut tissues. It can be noted that the pharynx, gut lumen, and body cavity were not stained with either antibody tested. | ||
| Wild type embryos and larvae were probed with a PDI-3-specific polyclonal antibody in combination with a DPY-7-specific monoclonal antibody. DPY-7 is a cuticle collagen that is expressed solely in the hypodermis and is localized in the cuticular ECM from the elongated embryo stage onwards. In the 2-fold embryo (~500 min after fertilization), PDI-3 staining was observed in most tissues including the gut and the hypodermis. Conversely, DPY-7 localized exclusively in the hypodermal cells. | |||
| Picture | WBPicture0000008219 | ||
| Reference | WBPaper00005704 | ||
| Antibody_info | WBAntibody00000601 |
