WormBase Tree Display for Expr_pattern: Expr1917
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| Expr1917 | Expression_of | Gene | WBGene00000803 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000803 | ||
| Homol | Homol_homol | F53H4:Expr | |
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| WBls:0000015 | |||
| WBls:0000021 | |||
| WBls:0000010 | |||
| WBls:0000013 | |||
| Anatomy_term (11) | |||
| Type | In_situ | Digoxigenin-11-dUTP (Roche)-labeled antisense (or sense) DNA (nt 1^381 in the complete cDNA sequence of the Cecsb gene). | |
| Reporter_gene | |||
| Pattern | In situ: Since multicopy transgene expression is silent in the C. elegans germline, Cecsb gene expression in the gonad was detected by in situ mRNA hybridization instead of GFP expression. The antisense cDNA probe of Cecsb stained the whole region of the gonad including oocytes, while the sense probe did not produce any signal. | ||
| Reporter_gene Assay: GFP first appeared during the 50^100 cell stage and was detected throughout the embryonic development. GFP expression was also observed in all of the somatic cells up to the L3 larval stage. However, GFP expression was relatively stronger in pi and P lineage vulval cells at the L3 larval stage, where active cell division and dierentiation take place. At the L4 larval stage, GFP was relatively stronger in amphid and tail neurons, and vulva and somatic gonad cells than in other body regions. In adult hermaphrodites, the overall GFP expression weakened, but strong expression was still observed in intestine, head, and tail regions. In L4 and adult males, the tail region, including the spicule, protractor, and PC sensilla, showed intense fluorescence. | |||
| Reference | WBPaper00005346 | ||
| Transgene | WBTransgene00027751 |
