WormBase Tree Display for Expr_pattern: Expr1819
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| Expr1819 | Expression_of | Gene | WBGene00004393 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004393 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000018 | ||||
| WBls:0000019 | ||||
| WBls:0000016 | ||||
| WBls:0000017 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0005753 | Certain | ||
| WBbt:0005772 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | nuclei | |||
| Type | Reporter_gene | |||
| Pattern | Run is expressed in the lateral hypodermis and intestine of C. elegans. Reporter gene expression in embryos from all transformed lines was first detected exclusively in hypodermal seam cells at the bean stage of development. Reporter gene expression was observed in the nuclei of these cells, and expression persisted throughout embryogenesis. Expression of run in seam cell nuclei was detected up until the L3 stage. Between the late embryonic stage and L3 stage, expression was also apparent in gut cell nuclei, with the anterior ring of four cells (int-1) showing the highest level of expression. Expression of run in binucleated gut cells with large nuclei became evident at the L2 stage. At the L3 stage, expression of run in gut cells was significantly decreased except in those cells in the most anterior part of the intestine. Expression in seam cells remained virtually unchanged. By the L4 stage, expression of run was undetectable. | |||
| Remark | Endogenous run expression was confirmed by immunohistochemical staining with antibody raised against the Runt domain. The pattern of endogenous run expression was essentially identical to that of run::GFP in the embryonic and larval stages, suggesting that the run::GFP reporter gene could represent the expression of endogenous run. | |||
| Reference | WBPaper00005040 | |||
| Transgene | WBTransgene00027706 |
