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WormBase Tree Display for Expr_pattern: Expr1154

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Name Class

Expr1154Expression_of (2)
Expression_dataLife_stageWBls:0000002
Anatomy_term (43)
GO_termGO:0005634
Subcellular_localizationlin-36::GFP expression in all cases was localized to nuclei. The GFP reporter gene used in the construction of this reporter construct did not contain a nuclear localization sequence, but as mentioned above, the lin-36 coding region contains a potential nuclear localization sequence.
TypeReporter_gene
Patternlin-36::GFP was expressed in the nuclei of the P(3-8).p cells and their descendants during vulval cell determination, division and invagination. Similar observations have been made with an independently constructed lin-36::GFP reporter. Besides the P(3-8).p cells, many other cells expressed GFP in strains bearing this reporter construct. Most notably, neurons of the head, tail and ventral cord expressed lin-36::GFP throughout development, and sporadic fluorescence was infrequently observed in the germline. Very weak staining was observed in hypodermal and intestinal nuclei.
PictureWBPicture0000008021
RemarkThis lin-36::GFP reporter transgene rescued the Muv phenotype of a lin-36; lin-15(n767) strain, indicating that it was expressed in cells needed for lin-36 function.
ReferenceWBPaper00003632
TransgeneWBTransgene00000282