WormBase Tree Display for Expr_pattern: Expr1074
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| Expr1074 | Expression_of | Gene | WBGene00003738 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003738 | ||
| Expression_data (3) | |||
| Subcellular_localization | membranes | ||
| Type | Antibody | AntiNID-1 antiserum that reacts with all isoforms was used to localize the protein in whole animals. | |
| Pattern | NID-1 was first detected in embryos at the beginning of morphogenesis (lima stage) localized to body wall muscle cells. As embryos elongate strong NID-1 staining is seen around body wall muscle cells and diffuse stain begins to accumulate on the surfaces of the pharyngeal and intestinal primordia. Once the embryo has elongated to the 2-fold stage, NID-1 has localized to the basal face of the body wall muscles and shows strong accumulation on the surfaces of the pharyngeal, intestinal, and gonad primordia. In three- and fourfold stage embryos, NID-1 accumulates to higher levels and remains localized under the four body wall muscle quadrants and on the surfaces of the pharynx, intestine, and gonad. In L1 larvae the intensity of staining of body wall muscle, pharynx, and intestine appears reduced, and strong staining associated with the nerve ring becomes apparent. This pattern continues through the L2 and L3 larval stages, with the addition of stronger staining of the distal tip cells as they lead the growth of the gonad. In late L3 to L4 stage larvae particularly strong NID-1 accumulation is seen associated with the distal tip cells and the developing somatic structures of the gonad, the spermatheca, uterus, and vulva. Under the body wall muscles of larval and adult stage animals NID-1 is organized as punctate lines. These lines follow the rows of dense bodies within the muscle cells. NID-1 also accumulates strongly at the outer edges of the muscle quadrants and more weakly at the boundaries between muscle cells within each quadrant. Less organized NID-1 staining is seen in the regions between the body wall muscle quadrants, presumably associated with the epidermal basement membranes in these regions. NID-1 accumulates along the four sublateral nerves that run beneath the center of each muscle quadrant. The sublateral nerves extend along dorso- and ventrolateral tracts from the nerve ring in the anterior of the animal to near the middle of the animal where they turn further lateral to positions coincident with the lateral edges of the body wall muscle quadrants. NID-1 accumulation on these nerves is seen in larval and adult animals and is similar in intensity to the staining at the edges of the body wall muscle quadrants. Staining along the edges of the body wall muscle quadrants appears to be associated with the muscle edges rather than the nerves in these regions because the staining closely follows the edge of the muscles and it does not display the left/right asymmetry expected for the ventral and dorsal nerve cords. Less organized NID-1 staining is also present in the regions between body wall muscle quadrants. | ||
| Picture | WBPicture0000008676 | ||
| WBPicture0000008678 | |||
| Remark | lima stage (author) = bean embryo (wjc). | ||
| Reference | WBPaper00004414 | ||
| Antibody_info | WBAntibody00000327 |
