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WormBase Tree Display for Expr_pattern: Expr10699

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Name Class

Expr10699Expression_ofGeneWBGene00002285
Reflects_endogenous_expression_ofWBGene00002285
Expression_dataLife_stageWBls:0000024
WBls:0000038
WBls:0000027
WBls:0000035
WBls:0000041
Anatomy_termWBbt:0005733Certain
WBbt:0005741Certain
WBbt:0005753Certain
WBbt:0005772Certain
WBbt:0005813Certain
WBbt:0005829Certain
WBbt:0006749Certain
WBbt:0006751Certain
WBbt:0006761Certain
WBbt:0007809Certain
TypeReporter_gene
Patternlet-7 primary transcripts are first detected at the end of the first larval stage and exhibit pulses of expression near the molt of each larval stage. Both of the plet-7::GFP constructs drove temporally regulated expression, initially observed at the end of the first larval stage (L1). Starting in late L1 (~10 h at 25°C), robust GFP was seen in muscle and neuronal cells in the head, including the nerve ring. Midway through L2, GFP accumulated in the neurons and muscle cells of the tail, body wall muscle (BWM), and ventral nerve cord (VNC). Beginning in L3, all transgenic lines began to express GFP in vulva precursor cells. After the onset of GFP expression in a particular tissue, it was maintained until adults became gravid with embryos (~52 h at 25° C). GFP was not detected in the germ line. Although plet-7A::GFP and plet-7B::GFP transgenics showed similar spatio-temporal expression patterns in most cell types, they were not entirely overlapping. In hypodermal and seam cells, the plet-7B::GFP reporter produced a robust GFP signal at the L3 stage. However, plet-7A::GFP animals exhibited only weak, transient expression of GFP in the hypodermal cells beginning at the L3 stage. Early in the second stage of development, all intestinal cells showed high expression of GFP in plet-7B::GFP transgenic animals, while GFP was undetectable in the intestine of plet-7A::GFP transgenic animals.
Remarkplet-7A::GFP and plet-7B::GFP expression differs in intestine, hypodermal, and seam cells.
ReferenceWBPaper00041775
TransgeneWBTransgene00031420