WormBase Tree Display for Expr_pattern: Expr10101
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| Expr10101 | Expression_of | Gene | WBGene00000931 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000931 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0004575 | Certain | ||
| WBbt:0004576 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005784 | Certain | |||
| WBbt:0006849 | Certain | |||
| WBbt:0008378 | Certain | |||
| GO_term | GO:0005730 | |||
| GO:0005654 | ||||
| Subcellular_localization | Every germline nucleus displays a single large DAO-5-positive nucleolus. In the 2-cell stage embryo, the DAO-5 signal consists of 15-25 small dots of varying intensity distributed throughout each nucleus. Nucleolus-like structures were never observed at this early stage. The first appearance of a nucleolar structure occurs at the 6- to 8-cell stage, when larger spherical bodies (~0.5 mm in diameter) are immunodetected amidst the dot-like signals. Shortly after, the number of small dot-like signals that are detected decreases drastically (but some persist until later stages) and, except for the germline cell, all embryonic nuclei then harbor one or, in the vast majority, two distinct DAO- 5 or FIB-1 signals of equal size and intensity. This pattern remains broadly similar throughout embryogenesis, with the notable exception that intestinal nuclei often contain a single large DAO-5/FIB-1 positive nucleolus at later stages. It should be noted that at all embryonic stages examined, a substantial pool of diffuse nucleoplasmic DAO-5 was also detected. In the adult, the localization of DAO-5 was examined in more detail in the polyploid intestinal nuclei, which contain a large centrally-located nucleolus. In these nucleoli, the DAO-5 signal describes a hollow sphere that surrounds a DNA-poor core. In addition to being present in this portion of the nucleolus, DAO-5 is found in numerous small punctate structures throughout the nucleoplasm of intestinal nuclei. The DAO-5/FIB-1 positive nucleoplasmic foci that we observe in early C. elegans embryos are reminiscent of the prenucleolar bodies that are detected in the last stages of mitosis and that coalesce on rDNA in early G1. | |||
| Type | Antibody | |||
| Picture | WBPicture0000012769 | |||
| WBPicture0000012770 | ||||
| WBPicture0000012771 | ||||
| WBPicture0000012773 | ||||
| WBPicture0000012774 | ||||
| WBPicture0000012775 | ||||
| WBPicture0000012776 | ||||
| WBPicture0000012777 | ||||
| Remark | Temporal description | |||
| Reference | WBPaper00041268 | |||
| Antibody_info | WBAntibody00002179 |
