WormBase Tree Display for Expr_pattern: Expr10098
expand all nodes | collapse all nodes | view schema
| Expr10098 | Expression_of | Gene | WBGene00003812 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003812 | |||
| Expression_data | Anatomy_term | WBbt:0005460 | Certain | |
| WBbt:0005772 | Certain | |||
| WBbt:0005813 | Certain | |||
| WBbt:0006925 | Certain | |||
| GO_term | GO:0016323 | |||
| GO:0045178 | ||||
| Type | Reporter_gene | |||
| Pattern | Using transcriptional reporters, we found that the nrf-5 promoter (Pnrf-5) drove expression in body wall muscle cells. We also generated NRF-5 translational fusions with or without the secretion signal (Pnrf-5ssGFP::mNRF-5 and Pnrf-5GFP::mNRF-5). Secreted GFP::mNRF-5 (ssGFP::mNRF-5) displayed a diffuse staining pattern and associated with the basal surface of pharyngeal cells and the basal-lateral membranes of the intestine, whereas nonsecretory GFP::mNRF-5 labeled body wall muscle cells but not the intestine. These results suggest that NRF-5 is synthesized in and secreted from muscle cells to recognize apoptotic cells and associate with the basal surface of pharyngeal and intestine cells. | |||
| Picture | WBPicture0000012744 | |||
| WBPicture0000012745 | ||||
| Reference | WBPaper00041220 | |||
| Transgene | WBTransgene00015228 | |||
| WBTransgene00015229 | ||||
| WBTransgene00015230 |
