WormBase Tree Display for Construct: WBCnstr00012243
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WBCnstr00012243 | Public_name | pKS2.15 | |
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Other_name | Expr4785_Ex | ||
Summary | Punc-46::GFP-ntx] | ||
Driven_by_gene | WBGene00006782 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | KS2.15 was constructed using the GFP and unc-54 terminator sequence contained in pPD95.85. The GFP sequence was amplified with primers containing PinAI sites on both sides. The PinAI sites were engineered so that they were in frame to the endogenous PinAI site within the first exon of the unc-46 gene and contained the ATG start codon from GFP. The amplified GFP and unc-54 terminator was cloned into the PinAI site in the full-length rescuing subclone for unc-46. A GFP product should be produced that is fused with 24 amino acids of UNC-46 protein at the N terminus. | ||
Used_for | Transgene_construct | WBTransgene00012603 | |
Reference | WBPaper00030788 |