WormBase Tree Display for Construct: WBCnstr00011590
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| WBCnstr00011590 | Other_name | Expr3672_Ex | |
|---|---|---|---|
| Summary | [nep-1::gfp] | ||
| Driven_by_gene | WBGene00013926 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | [nep-1::gfp] transcriptional fusion constructs. To analyse the expression pattern of nep-1, 1.2 kb and 4.0 kb of promoter were amplified by PCR, using the sense primers 5$(B!l(B-CCCAAGCTTGTGTACACCTCTTGTACGTCC-3$(B!l(B and 5$(B!l(B-GACCTGCAGACAGTTTTCATGTCGGCAATCA-3$(B!l(B, respectively and a common antisense primer (5$(B!l(B-CCCAAGCTTCTTACAGGTGTAGTAAGTAGGC-3$(B!l(B). The PCR-products and the vector pPD95.75 were digested with HindIII/PstI and ligated. The resulting plasmid (the GFP sequence under the control of the nep-1 promoter) was microinjected into young adult N2 wild-type C. elegans. Both nep-1 promoter constructs induced a strong green fluorescent protein (GFP) signal, suggesting that all essential regulatory elements are located within 1.2 kb upstream of the predicted start codon. --precise ends. | ||
| Clone | pPD95.75 | ||
| Used_for | Transgene_construct | WBTransgene00028807 | |
| Reference | WBPaper00026731 |
