WormBase Tree Display for Construct: WBCnstr00010221
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| WBCnstr00010221 | Other_name | Expr1329_Ex | |
|---|---|---|---|
| Summary | [unc-42::gfp] | ||
| Driven_by_gene | WBGene00006778 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | [unc-42::gfp] transcriptional fusions. The pRB18 construct contains 2.6 kb of upstream unc-42 genomic sequence fused to the GFP coding sequence at the N terminus of unc-42. To construct pRB18, a new XbaI site was introduced by PCR at the initiation codon of unc-42. An 80 bp sequence generated by digesting the PCR product with SalI and XbaI was co-ligated with a 2.5 kb HindIII-SalI genomic fragment into the HindIII-XbaI site of the GFP expression vector pPD95.77. pRB22 contains 7.5 kb of unc-42 genomic sequence and encodes a full-length UNC-42 protein with a GFP tag at the C terminus. pRB22 was generated by reconstructing the unc-42 genomic region in pRB18. A new XbaI site was inserted at the 3 end of the unc-42 coding sequence by PCR. | ||
| Used_for | Transgene_construct | WBTransgene00027466 | |
| Reference | WBPaper00003555 |
