WormBase Tree Display for Construct: WBCnstr00006614
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WBCnstr00006614 | Summary | [Punc-129::wrk-1, Punc-47::wrk-1] | |
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Driven_by_gene (2) | |||
Gene | WBGene00006942 | ||
Construction_summary | pTB118 was generated by cloning a Cfr9I/Bsp120I fragment from pTB111 into pTB116 (punc-129::gfp). pTB116 was generated by cloning a PCR fragment amplified from genomic DNA via oTB155 5'-AAACTGCAGAAACATGATATCGACGGAC-3' and oTB156 5'-TTTCCCGGGTTTCTTGCTTGCTCTTCC-3' into a XmaI/PstI fragment from pPD95.75. pTB112 was generated by cloning a XmaI/ApaI fragment from pTB111 into punc47::gfp. punc-47::gfp was generated by cloning the promoter sequence of the unc-47 gene spanning from 21190 bp to 27 bp relative to its ATG into pPD95.75. pTB111 was generated by cloning two PCR fragments into a pPD95.75 EcoRI/ PstI fragment: (1) oTB140 5'-AAACTGCAGCTTTAAAAAGTGTCAGACGATC-3' and oTB142 5' -GACGACTTCTGTGTTCTTCCTCC-3'. pTB111 was generated by cloning two PCR fragments into a pPD95.75 EcoRI/ PstI fragment: (1) oTB140 5'-AAACTGCAGCTTTAAAAAGTGTCAGACGATC-3' and oTB142 5'-GACGACTTCTGTGTTCTTCCTCC-3' were used to amplify the same promoter sequence present #1; (2) oTB143 5'-AAACCCGGGATGATGAAATTGGTTCTGCTG-3' and oTB136 5'-TTTGAATTCTTAAAATAAAAGATACATTGTGAC-3' were used to amplify the wrk-1b cDNA from yk498f3. | ||
Used_for | Transgene_construct | WBTransgene00006774 | |
Reference | WBPaper00028566 |