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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00056165:mina-1(ok1521)_downregulated_transcript

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  • Overview

    WBPaper00056165:mina-1(ok1521)_downregulated_transcript

    Species:
    Caenorhabditis elegans
    WormBase ID:
    WBPaper00056165:mina-1(ok1521)_downregulated_transcript
    Transcripts that showed significantly decreased expression in mina-1(ok1521) comparing to in N2.

    Algorithm:

    EdgeR; FDR > 0.1 and log2FC >0.7

    Remarks:

  • Associations

    Anatomy Terms:
    Life Stages:
    Life Stages Definition
    1-day post-L4 adult hermaphrodite CeAt 20 Centigrade: 24-48 hours after L4-adult molt. 4-5 days after first cleavage.
    GO terms:
    Processes:

  • Regulation

    Regulated by Gene:
    mina-1
    Regulated by Treatment:
    Regulated by Molecule:

  • References

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      • Journal article

      1

    1 reference found
    MINA-1 and WAGO-4 are part of regulatory network coordinating germ cell death and RNAi in C. elegans.
    Journal article
    Cell Death Differ
    2019

    Post-transcriptional control of mRNAs by RNA-binding proteins (RBPs) has a prominent role in the regulation of gene expression. RBPs interact with mRNAs to control their biogenesis, splicing, transport, localization, translation, and stability. Defects in such regulation can lead to a wide range of human diseases from neurological disorders to cancer. Many RBPs are conserved between Caenorhabditis elegans and humans, and several are known to regulate apoptosis in the adult C. elegans germline. How these RBPs control apoptosis is, however, largely unknown. Here, we identify mina-1(C41G7.3) in a RNA interference-based screen as a novel regulator of apoptosis, which is exclusively expressed in the adult germline. The absence of MINA-1 causes a dramatic increase in germ cell apoptosis, a reduction in brood size, and an impaired P granules organization and structure. In vivo crosslinking immunoprecipitation experiments revealed that MINA-1 binds a set of mRNAs coding for RBPs associated with germ cell development. Additionally, a system-wide analysis of a mina-1 deletion mutant compared with wildtype, including quantitative proteome and transcriptome data, hints to a post-transcriptional regulatory RBP network driven by MINA-1 during germ cell development in C. elegans. In particular, we found that the germline-specific Argonaute WAGO-4 protein levels are increased in mina-1 mutant background. Phenotypic analysis of double mutant mina-1;wago-4 revealed that contemporary loss of MINA-1 and WAGO-4 strongly rescues the phenotypes observed in mina-1 mutant background. To strengthen this functional interaction, we found that upregulation of WAGO-4 in mina-1 mutant animals causes hypersensitivity to exogenous RNAi. Our comprehensive experimental approach allowed us to describe a phenocritical interaction between two RBPs controlling germ cell apoptosis and exogenous RNAi. These findings broaden our understanding of how RBPs can orchestrate different cellular events such as differentiation and death in C. elegans.

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