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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00056090:E.faecalis_upregulated_hpx-2(dg047)

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  • Overview

    WBPaper00056090:E.faecalis_upregulated_hpx-2(dg047)

    Species:
    Caenorhabditis elegans
    WormBase ID:
    WBPaper00056090:E.faecalis_upregulated_hpx-2(dg047)
    Transcripts that showed significantly increased expression in hpx-2(dg047) after animals were exposed to E. faecalis OG1RF for 16 hours comparing to exposure to E. Coli OP50.

    Algorithm:

    Cuffcompare and Cuffdiff

    Remarks:

    Type: Bacteria Infection

  • References

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      • Journal article

      1

    1 reference found
    Heme peroxidase HPX-2 protects Caenorhabditis elegans from pathogens.
    Journal article
    PLoS Genet
    2019

    Heme-containing peroxidases are important components of innate immunity. Many of them functionally associate with NADPH oxidase (NOX)/dual oxidase (DUOX) enzymes by using the hydrogen peroxide they generate in downstream reactions. Caenorhabditis elegans encodes for several heme peroxidases, and in a previous study we identified the ShkT-containing peroxidase, SKPO-1, as necessary for pathogen resistance. Here, we demonstrated that another peroxidase, HPX-2 (Heme-PeroXidase 2), is required for resistance against some, but not all pathogens. Tissue specific RNA interference (RNAi) revealed that HPX-2 functionally localizes to the hypodermis of the worm. In congruence with this observation, hpx-2 mutant animals possessed a weaker cuticle structure, indicated by higher permeability to a DNA dye, but exhibited no obvious morphological defects. In addition, fluorescent labeling of HPX-2 revealed its expression in the pharynx, an organ in which BLI-3 is also present. Interestingly, loss of HPX-2 increased intestinal colonization of E. faecalis, suggesting its role in the pharynx may limit intestinal colonization. Moreover, disruption of a catalytic residue in the peroxidase domain of HPX-2 resulted in decreased survival on E. faecalis, indicating its peroxidase activity is required for pathogen resistance. Finally, RNA-seq analysis of an hpx-2 mutant revealed changes in genes encoding for cuticle structural components under the non-pathogenic conditions. Under pathogenic conditions, genes involved in infection response were differentially regulated to a greater degree, likely due to increased microbial burden. In conclusion, the characterization of the heme-peroxidase, HPX-2, revealed that it contributes to C. elegans pathogen resistance through a role in generating cuticle material in the hypodermis and pharynx.

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  • Associations

    Anatomy Terms:
    Life Stages:
    Life Stages Definition
    1-day post-L4 adult hermaphrodite CeAt 20 Centigrade: 24-48 hours after L4-adult molt. 4-5 days after first cleavage.
    GO terms:
    Processes:

  • Regulation

    Regulated by Gene:
    Regulated by Treatment:
    Bacteria infection: Enterococcus faecalis OG1RF. Exposure for 16 hours.
    Regulated by Molecule: