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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00053321:embryo-to-L1_upregulated_nos-1(gv5)nos-2(RNAi)_SMARTseq

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  • Comments on WBPaper00053321:embryo-to-L1_upregulated_nos-1(gv5)nos-2(RNAi)_SMARTseq (0)

  • Overview

    WBPaper00053321:embryo-to-L1_upregulated_nos-1(gv5)nos-2(RNAi)_SMARTseq

    Species:
    Caenorhabditis elegans
    WormBase ID:
    WBPaper00053321:embryo-to-L1_upregulated_nos-1(gv5)nos-2(RNAi)_SMARTseq
    Transcripts that showed significantly increased expression in L1 larva comparing to in embryo, in primordial germ cells (PGCs) of nos-1(gv5)nos-2(RNAi) animals, according to SMARTseq.

    Algorithm:

    For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses.

    Remarks:

  • References

    • Filter by article type
      • Journal article

      1

    1 reference found
    Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B.
    Journal article
    Elife
    2017

    Nanos RNA-binding proteins are required for germline development in metazoans, but the underlying mechanisms remain poorly understood. We have profiled the transcriptome of primordial germ cells (PGCs) lacking the nanos homologs nos-1 and nos-2 in C. elegans. nos-1nos-2 PGCs fail to silence hundreds of transcripts normally expressed in oocytes. We find that this misregulation is due to both delayed turnover of maternal transcripts and inappropriate transcriptional activation. The latter appears to be an indirect consequence of delayed turnover of the maternally-inherited transcription factor LIN-15B, a synMuvB class transcription factor known to antagonize PRC2 activity. PRC2 is required for chromatin reprogramming in the germline, and the transcriptome of PGCs lacking PRC2 resembles that of nos-1nos-2 PGCs. Loss of maternal LIN-15B restores fertility to nos-1nos-2 mutants. These findings suggest that Nanos promotes germ cell fate by downregulating maternal RNA and proteins that would otherwise interfere with PRC2-dependent reprogramming of PGC chromatin.

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  • Associations

    Anatomy Terms:
    Anatomy term Definition
    germline precursor cellmember of a set of two cells that generate exclusively sperm and ovum.
    Life Stages:
    Life Stages Definition
    L1 larva CeThe first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.
    embryo CeThe whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.
    GO terms:
    Processes:

  • Regulation

    No regulation data for WBPaper00053321:embryo-to-L1_upregulated_nos-1(gv5)nos-2(RNAi)_SMARTseq
    Regulated by Gene:
    Regulated by Treatment:
    Regulated by Molecule: