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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00047021:MWCNT_upregulated

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  • Overview

    WBPaper00047021:MWCNT_upregulated

    Species:
    Caenorhabditis elegans
    Attribute of Microarray experiment:
    WBPaper00047021:0hr_MWCNT-exposure_rep1
    WBPaper00047021:24hr_MWCNT-exposure_rep1
    WBPaper00047021:4hr_MWCNT-exposure_rep1
    WormBase ID:
    WBPaper00047021:MWCNT_upregulated
    Genes that showed significantly increased expression after exposed to multiwall carbon nanotubes (MWCNTs) for 4 and 24 h.

    Algorithm:

    N.A.

    Remarks:

  • Associations

    Anatomy Terms:
    Life Stages:
    Life Stages Definition
    1-day post-L4 adult hermaphrodite CeAt 20 Centigrade: 24-48 hours after L4-adult molt. 4-5 days after first cleavage.
    GO terms:
    Processes:

  • Regulation

    Regulated by Gene:
    Regulated by Treatment:
    Regulated by Molecule:
    multi-walled carbon nanotube

  • References

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      • Journal article

      1

    1 reference found
    A systems toxicology approach on the mechanism of uptake and toxicity of MWCNT in Caenorhabditis elegans.
    Journal article
    Chem Biol Interact
    2015

    The increased volumes of carbon nanotubes (CNTs) being utilized in industrial and biomedical processes carries with it an increased risk of unintentional release into the environment, requiring a thorough hazard and risk assessment. In this study, the toxicity of pristine and hydroxylated (OH-) multiwall CNTs (MWCNTs) was investigated in the nematode Caenorhabditis elegans using an integrated systems toxicology approach. To gain an insight into the toxic mechanism of MWCNTs, microarray and proteomics were conducted for C. elegans followed by pathway analyses. The results of pathway analyses suggested endocytosis, phagocytosis, oxidative stress and endoplasmic reticulum (ER) stress, as potential mechanisms of uptake and toxicity, which were subsequently investigated using loss-of-function mutants of genes of those pathways. The expression of phagocytosis related genes (i.e. ced-10 and rab-7) were significantly increased upon exposure to OH-MWCNT, concomitantly with the rescued toxicity by loss-of-function mutants of those genes, such as ced-10(n3246) and rab-7(ok511). An increased sensitivity of the hsp-4(gk514) mutant by OH-MWCNT, along with a decreased expression of hsp-4 at both gene and protein level suggests that MWCNTs may affect ER stress response in C. elegans. Collectively, the results implied phagocytosis to be a potential mechanism of uptake of MWCNTs, and ER and oxidative stress as potential mechanisms of toxicity. The integrated systems toxicology approach applied in this study provided a comprehensive insight into the toxic mechanism of MWCNTs in C. elegans, which may eventually be used to develop an "Adverse Outcome Pathway (AOP)", a recently introduced concept as a conceptual framework to link molecular level responses to higher level effects.

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