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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00044448:CholesterolStarvation_upregulated

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  • Overview

    WBPaper00044448:CholesterolStarvation_upregulated

    Species:
    Caenorhabditis elegans
    WormBase ID:
    WBPaper00044448:CholesterolStarvation_upregulated
    proteins that showed increased expression levels in worms grown in CS (Cholesterol Starvation) according to 2D-gel electrophoresis.

    Algorithm:

    Three pairs of gels were analyzed and variance analysis of spot volume and comparisons of mean values of samples were performed using SAS statistical software, with a significance level of p < 0.05. More than 1.5-fold differentially expressed proteins were analyzed by MALDI-TOF MS. The results from MS were further analyzed using MASCOT. Proteins with a score exceeding more than 67 and expression levels more than 2.2-fold difference were selected

    Remarks:

  • Regulation

    Regulated by Gene:
    Regulated by Treatment:
    Regulated by Molecule:
    cholesterol

  • References

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      • Journal article

      1

    1 reference found
    Cholesterol-responsive metabolic proteins are required for larval development in Caenorhabditis elegans.
    Journal article
    Mol Cells
    2013

    Caenorhabditis elegans, a cholesterol auxotroph, showed defects in larval development upon cholesterol starvation (CS) in a previous study. To identify cholesterol-responsive proteins likely responsible for the larval arrest upon CS, a comparative proteomic analysis was performed between C. elegans grown in normal medium supplemented with cholesterol (CN) and those grown in medium not supplemented with cholesterol (cholesterol starvation, CS). Our analysis revealed significant change (more than 2.2-fold, p &lt; 0.05) in nine proteins upon CS. Six proteins were down-regulated [CE01270 (EEF-1A.1), CE08852 (SAMS-1), CE11068 (PMT-2), CE09015 (ACDH-1), CE12564 (R07H5.8), and CE09655 (RLA-0)], and three proteins were up-regulated [CE29645 (LEC-1), CE16576 (LEC-5), and CE01431 (NEX-1)]. RNAi phenotypes of two of the down-regulated genes, R07H5.8 (adenosine kinase) and rla-0 (ribosomal protein), in CN were similar to that of larval arrest in CS, and RNAi of a down-regulated gene, R07H5.8, in CS further enhanced the effects of CS, suggesting that down-regulation of these genes is likely responsible for the larval arrest in CS. All three up-regulated genes contain putative DAF-16 binding sites and mRNA levels of these three genes were all decreased in daf-16 mutants in CN, suggesting that DAF-16 activates expression of these genes.

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  • Associations

    Anatomy Terms:
    Life Stages:
    Life Stages Definition
    1-day post-L4 adult hermaphrodite CeAt 20 Centigrade: 24-48 hours after L4-adult molt. 4-5 days after first cleavage.
    GO terms:
    Processes: