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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00041257:irradiation_upregulated

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  • Overview

    WBPaper00041257:irradiation_upregulated

    Species:
    Caenorhabditis elegans
    WormBase ID:
    WBPaper00041257:irradiation_upregulated
    Genes that showed significantly increased expression in mass spectrometry after 125 Gy of ionizing irradiation.

    Algorithm:

    Proteins with > 1.5 fold change in expression were considered differentially expressed.

    Remarks:

  • References

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      • Journal article

      1

    1 reference found
    Quantitative proteome analysis reveals RNA processing factors as modulators of ionizing radiation-induced apoptosis in the C. elegans germline.
    Journal article
    J Proteome Res
    2012

    The nematode Caenorhabditis elegans is an organism most recognized for forward and reverse genetic and functional genomic approaches. Proteomic analyses of DNA damage-induced apoptosis have not been shown because of a limited number of cells undergoing apoptosis. We applied mass spectrometry-based quantitative proteomics to evaluate protein changes induced by ionizing radiation (IR) in isolated C. elegans germlines. For this purpose, we used isobaric peptide termini labeling (IPTL) combined with the data analysis tool IsobariQ, which utilizes MS/MS spectra for relative quantification of peak pairs formed during fragmentation. Using stringent statistical critera, we identified 48 proteins to be significantly up- or down-regulated, most of which are part of a highly interconnected protein-protein interaction network dominated by proteins involved in translational control. RNA-mediated depletion of a selection of the IR-regulated proteins revealed that the conserved CAR-1/CGH-1/CEY-3 germline RNP complex acts as a novel negative regulator of DNA-damage induced apoptosis. Finally, a central role of nucleolar proteins in orchestrating these responses was confirmed as the H/ACA snRNP protein GAR-1 was required for IR-induced apoptosis in the C. elegans germline.

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  • Regulation

    Regulated by Gene:
    Regulated by Treatment:
    125 Gy ionizing radiation
    Regulated by Molecule:

  • Associations

    Anatomy Terms:
    Life Stages:
    Life Stages Definition
    1-day post-L4 adult hermaphrodite CeAt 20 Centigrade: 24-48 hours after L4-adult molt. 4-5 days after first cleavage.
    GO terms:
    Processes: