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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00040209:B.thuringiensis_downregulated_MY18

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  • Overview

    WBPaper00040209:B.thuringiensis_downregulated_MY18

    Species:
    Caenorhabditis elegans
    WormBase ID:
    WBPaper00040209:B.thuringiensis_downregulated_MY18
    Transcripts that showed significantly decreased expression after 8 hour of exposure of C.elegans strain MY18 to pathogenic B. thuringiensis B-18247.

    Algorithm:

    Differential gene expression was assessed with a mixed regression model, including pathogen as a fixed and array as a random factor using the restricted maximum likelihood (REML) approach. The pathogen effect was evaluated with an F3-test using a pooled estimator of the error-variance and comparison of the tabulated p-values with the F distribution rather than a permutation analysis, which was unsuitable for this study because of low sample size (maximum of four replicates). To correct for multiple testing authors adjusted the significance level with the help of the false discovery rate (FDR).

    Remarks:

    Type: Bacteria Infection
  • Regulation

    Regulated by Gene:
    Regulated by Treatment:
    Bacteria infection: Bacillus thuringiensis B-18247
    Regulated by Molecule:
  • References

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    • Journal article

      1

    1 reference found
    Protist-type lysozymes of the nematode Caenorhabditis elegans contribute to resistance against pathogenic Bacillus thuringiensis.
    Journal article
    PLoS One
    2011

    Pathogens represent a universal threat to other living organisms. Most organisms express antimicrobial proteins and peptides, such as lysozymes, as a protection against these challenges. The nematode Caenorhabditis elegans harbours 15 phylogenetically diverse lysozyme genes, belonging to two distinct types, the protist- or Entamoeba-type (lys genes) and the invertebrate-type (ilys genes) lysozymes. In the present study we characterized the role of several protist-type lysozyme genes in defence against a nematocidal strain of the Gram-positive bacterium Bacillus thuringiensis. Based on microarray and subsequent qRT-PCR gene expression analysis, we identified protist-type lysozyme genes as one of the differentially transcribed gene classes after infection. A functional genetic analysis was performed for three of these genes, each belonging to a distinct evolutionary lineage within the protist-type lysozymes (lys-2, lys-5, and lys-7). Their knock-out led to decreased pathogen resistance in all three cases, while an increase in resistance was observed when two out of three tested genes were overexpressed in transgenic lines (lys-5, lys-7, but not lys-2). We conclude that the lysozyme genes lys-5, lys-7, and possibly lys-2 contribute to resistance against B. thuringiensis, thus highlighting the particular role of lysozymes in the nematode's defence against pathogens.

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  • Associations

    Anatomy Terms:
    Life Stages:
    Life Stages Definition
    L4 larva CeThe fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.
    GO terms:
    Processes: