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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00034636:heat_shock_downregulated

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  • Overview

    WBPaper00034636:heat_shock_downregulated

    Species:
    Caenorhabditis elegans
    Attribute of Microarray experiment:
    WBPaper00034636:heat_control__1
    WBPaper00034636:heat_control__2
    WBPaper00034636:mild_heat_shock__1
    WBPaper00034636:mild_heat_shock__2
    WormBase ID:
    WBPaper00034636:heat_shock_downregulated
    Genes Showing Significant Expression Changes (P <= 0.05 After FDR) in Heat-Shocked (30 centigrade for 1.5 h; Two Arrays), Compared to Sham Controls (26 centigrade for 2.5 h; Five Arrays).

    Algorithm:

    paired t-tests

    Remarks:

    Type: Response to Temperature Stimulus - Heat

  • Regulation

    Regulated by Gene:
    Regulated by Treatment:
    heat shock
    Regulated by Molecule:

  • Associations

    Anatomy Terms:
    Life Stages:
    Life Stages Definition
    L4 larva CeThe fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.
    GO terms:
    Processes:

  • References

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      • Journal article

      1

    1 reference found
    Low-intensity microwave irradiation does not substantially alter gene expression in late larval and adult Caenorhabditis elegans.
    Journal article
    Bioelectromagnetics
    2009

    Reports that low-intensity microwave radiation induces heat-shock reporter gene expression in the nematode, Caenorhabditis elegans, have recently been reinterpreted as a subtle thermal effect caused by slight heating. This study used a microwave exposure system (1.0 GHz, 0.5 W power input; SAR 0.9-3 mW kg(-1) for 6-well plates) that minimises temperature differentials between sham and exposed conditions (&lt; or =0.1 degrees C). Parallel measurement and simulation studies of SAR distribution within this exposure system are presented. We compared five Affymetrix gene arrays of pooled triplicate RNA populations from sham-exposed L4/adult worms against five gene arrays of pooled RNA from microwave-exposed worms (taken from the same source population in each run). No genes showed consistent expression changes across all five comparisons, and all expression changes appeared modest after normalisation (&lt; or =40% up- or down-regulated). The number of statistically significant differences in gene expression (846) was less than the false-positive rate expected by chance (1131). We conclude that the pattern of gene expression in L4/adult C. elegans is substantially unaffected by low-intensity microwave radiation; the minor changes observed in this study could well be false positives. As a positive control, we compared RNA samples from N2 worms subjected to a mild heat-shock treatment (30 degrees C) against controls at 26 degrees C (two gene arrays per condition). As expected, heat-shock genes are strongly up-regulated at 30 degrees C, particularly an hsp-70 family member (C12C8.1) and hsp-16.2. Under these heat-shock conditions, we confirmed that an hsp-16.2::GFP transgene was strongly up-regulated, whereas two non-heat-inducible transgenes (daf-16::GFP; cyp-34A9::GFP) showed little change in expression.

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